DNA Q-Extraction Solution

The non-toxic DNA Q-Extraction Solution provides fast and easy extraction of nucleic acids from various mammalian tissues (e.g. mouse tails, ear snips, liver, kidney, lung), saliva and bacteria. The PCR-ready nucleic acid is extracted, using one tube and one reaction, in as little as 8 minutes.

The one-step lysis is performed in either a thermocycler or heating block and is divided into two simple heating steps. The extracted DNA is then ready for PCR without further handling such as vortex, centrifugation or dilutions. The obtained DNA extract is stable at -20°C for up to one week and at -80°C for long term storage.

Recent user data confirm that the DNA Q-Extraction Solution can also be successfully used with fungi samples, such as yeast and mold. The standard 8-minute protocol yields PCR-ready DNA without the need for additional clean-up or purification steps. This expands the range of applications and makes the DNA Q-Extraction Solution a versatile tool for rapid DNA extraction, even from more challenging sample types.

For optimal genotyping results we recommend to use our DNA Q-Extraction Solution together with our Red Mastermix Master Mix (2X) for PCR (M3029) or our SuperHot Mastermix Blue (2X) (M3007b). E.g. use 2µL to 5µL of the Q-Extract solution for a 25µL PCR reaction. 

For plant tissue we recommend using our specific Plant DNA Q-Extraction Solution (M3230).

Features:
- One-reagent set-up
- Minimal handling
- Rapid 8-minute protocol
- PCR-ready DNA
- no sample / yield loss
- DNA extracts from mammalian tissues and bacteria
- Non-toxic reagents
- Scalable set-up
- Automation-friendly

Extraction Protocol
Preparation of DNA extraction should be performed in a separate area from that used for setting up the PCR reaction.

1. Thaw DNA Q-Extraction Solution.
For the first time use, aliquot the DNA Q-Extraction solution into smaller volumes. (DNA Q-Extraction Solution has a cloudy appearance).
2. Add your sample to a tube containing 100µL DNA Q-Extraction Solution. Recommended sample sizes are shown in Table 1 of the data sheet.
3. Vortex the tube containing the sample and the DNA extraction solution for 15 sec. Make sure that the sample is completely covered by the DNA Q-Extraction Solution.
4. Transfer the tube to a heat block or a thermal cycler and incubate for

1. 65 °C for 6 min
2. 98 °C for 2 min
3. 4 °C (or cool down on ice)

The DNA extract is now ready for PCR.

Anwendungsbeispiele:

Efficient Amplification of DNA extracts from various mammalian tissue.

DNA Q-Extraction Solution was used together with our RedMastermix (M3029 >) to extract and amplify genomic DNA from various mammalian tissues. For DNA extraction, 0.5-10 mg tissue or 20µL salvia was added to 100µL of DNA Q-Extraction Solution.

M: DNA marker Iqon Low DNA Ladder. Lane 1-5: Different mouse tissues as depicted, GAPDH (266 bp). Lane 6: Chicken muscle tissue, HRPT1 (245 bp) and Lane 7: Human saliva, DMD17 (415 bp).

DNA Q-Exctract Solution works over a wide range of sample sizes

In order to investigate the flexibility of DNA Q-Extraction Solution protocol, varying amounts of chicken muscle tissues were added to 100µL of DNA Q-Extraction Solution. The extraction was conducted as specified in the datasheet for DNA Q-Extraction Solution. The extracted DNA was subjected to PCR using the Genaxxon RedMastermix (M3029 >). The results show that the DNA Q-Extraction protocol provides the user with a high degree of flexibility over a wide range of applied sample amount.

M: DNA marker Iqon Low DNA Ladder. Lanes 1-28: Varying amounts (mg) of chicken muscle tissue, HRPT1 (245 bp).