GENAzol - RNA Purification solution

GENAzol is a ready-to-use reagent for the isolation of high-quality total RNA or the simultaneous isolation of RNA, DNA and proteins from biological samples. The single-phase solution of aqueous guanidine isothiocyanate solution can be used to isolate RNA, DNA and proteins in various fractions from cell and tissue samples, e.g. from humans, animals, plants, yeasts, bacteria or viruses (usually within one hour).

Properties of GENAzol:
- Parallel isolation of RNA, DNA and protein from the same sample
- Superior suitability for lysis even with difficult samples
- Optimized formulations and protocols for tissues, cells, blood, viruses and bacteria.

Reliable purification of RNA from samples of different volumes and sources
GENAzol is also suitable for small amounts of tissue (50–100mg) and cells (5× 10E6) as well as for larger amounts of cells (>10E7) and contains protocols for the purification of RNA from human, animal, plant or bacterial Rehearse. GENAzol maintains the integrity of the RNA due to the very effective inhibition of RNase activity when cells and components are dissolved during sample homogenization. GENAzol enables the simultaneous processing of a large number of samples. The entire isolation step is usually completed in 1 hour. The total RNA isolated with GENAzol is free from protein and DNA contamination.

Procedure for the isolation of RNA, DNA and proteins
GENAzol enables sequential precipitation of RNA, DNA and proteins from a single sample. After homogenizing the sample with GENAzol, chloroform is added and the homogenate separates into a clear aqueous top layer (which contains RNA), a boundary layer and a organic bottom layer (with DNA and proteins). RNA is precipitated from the aqueous layer with isopropanol. DNA precipitation from the organic boundary layer takes place with ethanol. Finally, the protein from the phenol-ethanol supernatant is precipitated with isopropanol. Precipitated RNA, DNA or protein is washed to remove impurities and then resuspended for use in subsequent applications.

This is a dangerous good with UN number UN2821. Not for sale outside the European Union. UFI-Code: 7H2D-1Q71-Q00M-D0WU