Plant DNA Q-Extraction Solution

The non-toxic Plant DNA Q-Extraction Solution provides fast and easy extraction of nucleic acids from various plant material (e.g. ivy or stinging nettle). The PCR-ready nucleic acid is extracted, using one tube and one reaction, in as little as 8 minutes.

The one-step lysis is performed in either a thermocycler or heating block and is divided into two simple heating steps. The extracted DNA is then ready for PCR without further handling such as vortex, centrifugation or dilutions. The obtained DNA extract is stable at -20°C for up to one week and at -80°C for long term storage.

For optimal genotyping results we recommend to use our Plant DNA Q-Extraction Solution together with our InhibiCore Rapid Plant PCR MasterMix (5X). We also offer them as a complete InhibiCore Rapid Plant DNA Q-Extraction PCR Kit for fast direct PCR from plant material.

Features:
- One-reagent set-up
- Minimal handling
- Rapid 8-minute protocol
- PCR-ready DNA
- no sample / yield loss
- DNA extracts from plants
- Non-toxic reagents
- Scalable set-up
- Automation-friendly

Extraction Protocol
Preparation of DNA extraction should be performed in a separate area from that used for setting up the PCR reaction.

1. Thaw Plant DNA Q-Extraction Solution.
For the first time use, mix the Plant DNA Q-Extraction solution well and aliquote it into smaller volumes. (Plant DNA Q-Extraction Solution has a cloudy appearance).
2. Add your sample to a tube containing 100µL Plant DNA Q-Extraction Solution. Recommended sample sizes are shown in the table of the manual.
3. Vortex the tube containing the sample and the DNA extraction solution for 15 sec. Make sure that the sample is completely covered by the Plant DNA Q-Extraction Solution.
4. Transfer the tube to a heat block or a thermal cycler and incubate for

1. 65 °C for 6 min
2. 98 °C for 2 min
3. 4 °C (or cool down on ice)

The DNA extract is now ready for PCR. 
Please note: Plant DNA lysate should be diluted 5- to 10-fold prior to PCR. We recommend performing a serial dilution.

Applications examples:

1. Sensitive qPCR analysis of chlorophyll gene targets from wheat lysates

Reliable and sensitive detection of chlorophyll gene targets from plant lysates was demonstrated using qPCR with the InhibiCore Rapid Plant PCR MasterMix (5X) (M3250) and fluorescent dye chemistry.
A serial dilution of wheat lysate (10% to 0.001%) was used to evaluate sensitivity and inhibitor tolerance.

Successful amplification was achieved across all dilutions, confirming strong robustness against plant-derived inhibitors even at high lysate concentrations. A single, specific melt curve peak confirmed assay specificity with no non-specific amplification. Samples were prepared using the rapid 8-minute Plant DNA Q-Extraction Solution protocol, enabling fast DNA preparation without conventional purification steps.

Figure 1: qPCR data by wheat lysate qPCR with InhibiCore Rapid Plant PCR MasterMix (5X).
The figure shows qPCR amplification curves and melting curve analysis of a wheat lysate dilution series (10% to 0.001%) and a no-template control (NTC) using InhibiCore Rapid Plant PCR MasterMix (5X) and 1xGreenDye. Clear, concentration-dependent amplification is observed in the FAM channel, with reduced signal intensity at 10% lysate due to matrix effects. Melting curve analysis reveals a single specific peak for all positive samples, confirming specific target amplification without non-specific products.

2. Benchmark of Direct PCR performance from 10% tomato lysate

Figure 2: Plant direct PCR from plant samples Benchmark - inhibitor effect from Tomato lysate
Direct PCR was performed using a cycling protocol of 95 °C for 3 min, followed by 40 cycles of 95 °C for 10 sec and 60 °C for 30 sec, with subsequent melt curve analysis. InhibiCore Rapid Plant PCR MasterMix (5X) and Competitor N showed successful amplification, while Competitor T and Competitor Q were strongly inhibited and did not generate reliable amplification curves. InhibiCore Rapid Plant PCR MasterMix (5X) exhibited the earliest Ct value among all tested systems, indicating the highest tolerance to plant-derived PCR inhibitors and superior performance in crude tomato lysates.

3.  Easy sample handling