CentriPure P100 columns for protein purification
Advantages at a glance
- Sample volume: 10mL
- Removes up to 99.99% of low molecular weight impurities
- Sample / protein recovery: normally about 85%
- Size exclusion: about 5kDa
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Delivery time: 3-8 days
Product information "CentriPure P100 columns for protein purification"
CentriPure P100 columns are pre-hydrated gel filtration columns for protein purification and desalting. CentriPure P100 Gel Filtration Columns are designed for rapid and efficient removal of small molecules (salts, dyes, ammonia, haptens, biotin, etc.) from antibodies, enzymes and other proteins. The gel matrix of CentriPure is Zetadex-25, a beaded composite material developed by emp Biotech composed partially of polymerized dextran. It exhibits high selectivity, high resolution and chemical stability. Molecules purified with Zetadex-25 are separated according to size. Smaller molecules pass significantly slower through the column than larger molecules. Buffer and pH effects on resolution are minimal.
The size exclusion cut-off for Zetadex-25 is 5 kD for proteins. Proteins larger than 5 kD in a sample volume of up to 10mL can be purified with an elution volume of 12mL to 15mL.
Specifications:
Removes up to 99.99% of low molecular weight impurities
Sample / protein recovery: normally about 85%
Sample volume: 10mL
Size exclusion: about 5kDa
Process time: about 15 minutes
Bead diameter: 50 - 150µm
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