Ceramic beads for tissue lysis
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Product information "Ceramic beads for tissue lysis"
Ceramic beads for the lysis of tissue from a wide range of sample material, e.g. for RNA extraction. The kit contains 100 preps with 1.4mm ceramic beads (zirconia) and comes pre-proportioned to 1g in 2mL standard screw cap tubes. The beads are suitable for the homogenization of "soft" tissue from lung, liver, brain, kidney, spleen, skin, cells from cell culture, as well as for plant tissues such as e.g. Leaves or even whole insects. The usabl sample amount ranges from up to 200mg tissue, up to 200μL of cells suspended in water or isotonic saline solution, or up to 100mg wet weight of tissue culture cells grown in suspension (e.g., ≤10E7 mammalian cells).
The beads can be used for a variety of samples and applications, e.g. B. for veterinary diagnostics (BSE screening), pharmacology and toxicology (drug detection), criminology and forensic medicine, food industry (GMO detection), microbiology in the environment (microorganism lysis). as well as the isolation of nucleic acids and proteins. They are chemically inert and do not bind nucleic acids.
The beads are comparable with MP Biomedical No. 116913100 (equivalent to Lysing Matrix D) and are compatible with most commercially available homogenizers, like FastPrep® (MP Biomedicals), MagNA Lyser Instrument (Roche).
You can reorder our RNA > and DNA purification columns > for the isolation of total RNA or DNA from enzymatic reactions or ceramic beads > separately. These articles can also be used with buffers/kits from other suppliers.
With our HotScriptase RT PCR master mix >, you can quickly and easily transcribe and amplify RNA into DNA by normal PCR. You do not need a reverse transcriptase and no reverse transcription step.
Specifications:
RNase-free ceramic beads of 1.4mm diameter for disintegration of soft tissue, frozen tissue, plant tissue or insects.
up to 200mg tissue (fresh, frozen, dried, etc.)
up to 200µL cells suspended in water or isotonic saline solution
up to 100mg wet weight of tissue culture cells grown in suspension (up to 10E7 mammalian cells).
Composition of beads: ZrO2: 64% - SiO2: 33%
Colour of beads: slightly beige/yellowish.
Applikation / Application:
- veterinary diagnostics (BSE screening) - pharmacology and toxicology (drug detection) - criminology and forensic medicine - food industry (GMO detection) - microbiology in the environment (microorganism lysis) - isolation of nucleic acids and proteinsKlassifizierungen / Classification
Documents:
Product descriptionGeneral Data 1
General Data 3
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Category List
In order to isolate nucleic acids with the highest possible yield, cell disruption should be carried out as gently as possible. Specimen material such as seeds, spores, bones, skin, etc. are a particular challenge.
The ceramic beads from Genaxxon offer a reliable and effective method for fast cell disruption even of difficult sample material.
Particularly resistant samples such as bones and cartilage require high digestion energy - here are e.g. the ceramic beads from Genaxxon are well suited. For microorganisms
small glass beads are again more suitable in cultures. This method of variation was not available with cell lysis methods.
The ceramic beads from Genaxxon are suitable for use in bead tube homogenizers such as the Power LyzerTM 24, OMNI Bead Ruptor, FastPrep® (MP Biomedicals) or devices of a similar design. With a special attachment, cell lysis works extremely well with the Vortex Genie 2.
Application examples for the use of ceramic beads:
Liver / lung (2.8mm beads); Yeast (1.4mm beads); Seeds (2.8mm beads); Arabidopsis (2.8mm beads); Soil samples / stool samples (1.4mm beads), pine needles (2.8mm beads).
Source: NCBI PubMed
The Defense-Related Isoleucic Acid Differentially Accumulates in Arabidopsis Among Branched-Chain Amino Acid-Related 2-Hydroxy Carboxylic Acids
Rafał P. Maksym, Andrea Ghirardo, Wei Zhang, Veronica von Saint Paul, Birgit Lange, Birgit Geist, Mohammad-Reza Hajirezaei, Jörg-Peter Schnitzler, Anton R. Schäffner
Front Plant Sci. 2018; 9: 766.
doi: 10.3389/fpls.2018.00766
PMCID: PMC6002512
