Challenger Hotstart RT-qPCR 4x Mastermix
Advantages at a glance
- Room-temperature rxn setup – no cooling needed during pipetting
- warm-start feature of RTase – enables premixing of primers&probes
- Safe handling – includes dUTP/UDG for contamination control
- Ultra-fast performance – supports 5 min RT-steps and 30-min fast protocols
- High sensitivity – reliable detection down to single-digit -copy RNA copies
- Convenient handling – Single tube, 4x mix allows high multplexing setups
Product number:
M3060.0100
Ready to ship today,
Delivery time 1-2 workdays
Shipment: on wet ice. Store at -20°C. For laboratory usage only!
Ready to ship today,
Delivery time 1-2 workdays
Shipment: on wet ice. Store at -20°C. For laboratory usage only!
Product information "Challenger Hotstart RT-qPCR 4x Mastermix"
Why Choose Challenger Hotstart RT-qPCR Mix?
- Room-temperature setup – Easy pipetting without cooling, saving time and reducing errors.
- warm-start reverse transcriptase – Prevents nonspecific amplification and allows primer/probe premixing.
- Superior contamination control – UDG/dUTP system eliminates carryover contamination for reliable qPCR results.
- Fast RT-qPCR protocols – Complete assays in just 30 minutes with a 5-minute reverse transcription step.
- High sensitivity RT-qPCR – Detect as few as 1–10 RNA copies.
- Flexible applications – Single and multiplex RT-qPCR for viral detection, gene expression, and diagnostics.
Optimized for High-Performance qPCR Workflows
The 4× concentrated formulation allows high-throughput and multiplexing while keeping reaction efficiency high. The combination of warm-start RTase and antibody-modified Taq DNA polymerase and a RNAse inhibitor ensures precise control, so your reactions only start when you are ready.
Contamination-Free One-Step RT-qPCR
Built-in dUTP/UDG safeguards protect your assays from false positives due to carryover contamination. This, in combination with an RNase inhibitor is essential for labs running high-sensitivity viral RNA detection and diagnostic assay developments.
Fast RT-qPCR Protocols Without Sacrificing Accuracy
With Challenger, you can speed up detection while keeping sensitivity intact:
- Standard program: 55 °C for 15 min RT, ~1 hr total
- Fast program: 55 °C for 5 min RT, ~30 min total
Benchmark studies with HBV/HCV RNA samples confirmed superior stability and consistent Ct values compared to competitor brands.
Long-Term Stability You Can Trust
Unlike conventional RT-qPCR mixes, the Challenger Hotstart RT-qPCR Master Mix is engineered for long shelf life. Even under accelerated testing at 37 °C for 10 days, the mix maintained stable multiplex detection - ensuring reliable performance during storage and shipping.
Example Applications
- Viral RNA detection: HBV, HCV, Influenza, RSV, SARS-CoV-2, hMPV, PIV1, and more
- Gene expression analysis: accurate detection of high- and low-copy RNA targets
- Clinical research & diagnostics: robust, reproducible data
- High-throughput testing: multiplex RT-qPCR panels for respiratory, gastrointestinal, and hepatitis viruses
- Many more…
Key Features Summary
- One-step RT-qPCR Master Mix with warm-start RTase
- High sensitivity qPCR mix – detects single RNA copies
- Fast RT-qPCR protocol – 5 min RT, 30 min total
- Long stability RT enzyme – stable at 37 °C for 10 days
- UDG/dUTP contamination control – eliminates carryover risk
- 4× concentrated format – supports multiplexing
High sensitivity and stable detection
Stable detection of single-digit copy templates using the Challenger Hotstart RT-qPCR 4X Mastermix:
Specifications:
Ready-to-use 4-time RT-qPCR mix
with warm-start Reverse Transcriptase
with Hot Start Polymerase
with RNase Inhibitor
with dNTPs
with dUTP and UDG enzyme
without ROX®
Applikation / Application:
reverse transcription qPCR
Einheiten / Units:
Quelle / Source:
synthetic
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-05-02
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.
Documents:
Manuals
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Source: NCBI PubMed
Source: NCBI PubMed