HotScriptase Probe RT-qPCR Mastermix without ROX

HotScriptase Probe Mastermix for RT-PCR without an isothermal intermediate step, thereby saving time and money: HotScriptase Probe Mastermix was successfully used by several customers for the detection of RNA viruses like Zika or SARS-CoV-2 (COVID-19 detection). HotScriptase is a extremely thermostable reverse transcriptase and DNA polymerase in one enzyme.

HotScriptase Probe Mastermix enables the amplification of RNA or DNA target sequences with quick and easy PCR protocols, even without an isothermal reverse transcription step. The light blue color improves the visibility of the mixture during the pipetting steps, especially if white or transparent PCR plates or PCR tubes are used. 

Our Hotscriptase is insensitive to inhibitors, so that a PCR can be carried out directly from wastewater samples with the HotScriptase!

• faster: One-Step - NO time consuming isothermal intermediate step necessary.
• easier: reverse transcriptase and DNA polymerase in one single enzyme; reverse transcription and DNA amplification in parallel during the PCR elongation step. You need only a PCR protocol.
• saver: much reduced contamination risk, due to reduced pipetting steps.
• extreme thermostablility: secondary structures will be a minor problem due to higher temperatures of PCR compared to reverse transcription reactions.
• cheaper: parallel reverse transcription and amplification by HotScriptase RT saves time and costs.

The Genaxxon HotScriptase DNA polymerase for reverse transcription enables a "One-Step” RT-PCR directly from cell suspensions or mammals cells without an isothermal reverse transcription intermediate step. Just give cells or cell suspension (100 up to <10,000 cells) directly to the PCR reaction mixture, mix, place in a thermal cycler and start PCR program.

HotScriptase RT polymerase is a biotechnical engineered, extremely thermostable reverse transcriptase and combined DNA polymerase, obtained through directed, artificial evolution. The HotScriptase 2-time master mix is a ready-to-use mixture of the propietory HotScriptase, buffer, dNTPs and special components. The recommended amplicon size should be between 60-400 bp!

This master mix contains no ROX nor green dye!