HotScriptase RT Mastermix with GreenDye without ROX
Advantages at a glance
- Faster: One-step process—no time-consuming isothermal step.
- Easier: Single enzyme for RT and PCR—only a PCR protocol needed.
- Safer: Reduced contamination risk with fewer pipetting steps.
- More thermostable: Handles secondary structures better due to higher temperatures.
- Cost-effective: Saves time and costs with parallel RT and amplification.
Ready to ship today,
Delivery time 1-2 workdays
Shipment: on wet ice. Store at -20°C. For laboratory usage only!
HotScriptase RT Master Mix for One-Step RT-PCR without isothermal reverse transcription step. Using HotScriptase RT is very time and cost efficienct as HotScriptase RT is a reverse transcriptase and a DNA Polymerase combined in one single highly thermostable enzyme.
- faster: One-Step - NO time consuming isothermal intermediate step necessary.
- easier: reverse transcriptase and DNA polymerase in one single enzyme; reverse transcription and DNA amplification in parallel during the PCR elongation step. You need only a PCR protocol.
- saver: much reduced contamination risk, due to reduced pipetting steps.
- extreme thermostablility: secondary structures will be a minor problem due to higher temperatures of PCR compared to reverse transcription reactions.
- cheaper: parallel reverse transcription and amplification by HotScriptase RT saves time and costs.
The Genaxxon HotScriptase DNA polymerase for reverse transcription enables a "One-Step” RT-PCR directly from cell suspensions or mammals cells without an isothermal reverse transcription intermediate step.
HotScriptase RT polymerase is a biotechnical engineered, extremely thermostable reverse transcriptase and combined DNA polymerase, obtained through directed, artificial evolution. The HotScriptase 2-time master mix is a ready-to-use mixture of the propietory HotScriptase, buffer, dNTPs and special components. The recommended amplicon size should be between 60-400 bp!
This master mix contains a fluorescence dye but no ROX!
Specifications:
2-time master mix for direct qPCR from RNA without separate transcription step.
Master mix without ROX, with green fluorescence dye.
Aliquot size: 1.25mL for 100 x 25µL PCR reactions.
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-05-02
Documents:
ProtocolsManuals
General Data 1
General Data 2
General Data 3
Category List
Source: NCBI PubMed
Natural phenolic compounds as biofilm inhibitors of multidrug-resistant Escherichia coli - the role of similar biological processes despite structural diversity
David Buchmann, Michael Schwabe, Romano Weiss, Andreas W. Kuss, Katharina Schaufler, Rabea Schlüter, Stefan Rödiger, Sebastian Guenther, Nadin Schultze
Front Microbiol. 2023 Sep; 14:1232039.
doi: 10.3389/fmicb.2023.1232039.
PMCID: PMC10507321.
