HotScriptase RT polymerase
Advantages at a glance
- Faster: One-step process—no time-consuming isothermal step.
- Easier: Single enzyme for RT and PCR—only a PCR protocol needed.
- Safer: Reduced contamination risk with fewer pipetting steps.
- More thermostable: Handles secondary structures better due to higher temperatures.
- Cost-effective: Saves time and costs with parallel RT and amplification.
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.
Shipment: on wet ice. Store at -20°C. For laboratory usage only!
"One-Step" RT-PCR without isothermal reverse transcription step. Using HotScriptase RT is very time and cost efficient as HotScriptase RT is a reverse transcriptase and a DNA polymerase combined in one highly thermostable enzyme. (find out more about our realtime master mixes >)
- faster: One-Step - NO time consuming isothermal intermediate step necessary.
- easier: reverse transcriptase and DNA polymerase in one single enzyme; reverse transcription and DNA amplification in parallel during the PCR elongation step. You need only a PCR protocol.
- saver: much reduced contamination risk, due to reduced pipetting steps.
- extreme thermostability: secondary structures will be a minor problem due to higher temperatures of PCR compared to reverse transcription reactions.
- cheaper: parallel reverse transcription and amplification by HotScriptase RT saves time and costs.
The Genaxxon bioscience HotScriptase can be ordered as a 50x concentrated solution of the DNA polymerase or as a convenient 2-time RT master mix >. The 2-time RT master mix contains all necessary ingredients for RT-PCR without specific primers, probes or RNA/DNA. Additionally, Genaxxon offers the 2-time RT master mix with Green Dye.
HotScriptase RT Polymerase is an biotechnical engineered, extremely thermostable reverse transcriptase and combined DNA polymerase, obtained through directed, artificial evolution.
Recommended amplicon size should be between 60-400 bp!
Specifications:
50x concentrated solution
for direct PCR from RNA
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-05-02
Documents:
ManualsGeneral Data 1
General Data 3
Category List
Source: NCBI PubMed
HotScriptase is only working with specific primers! We highly recommend to design primers with an annealing temperature >65°C.
Our HotScriptase may fail if you use your normally designed primers for RT-PCR.
