M-MuLV Reverse Transcriptase RNase H negative
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Product information "M-MuLV Reverse Transcriptase RNase H negative"
The Genaxxon M-MuLV Reverse Transcriptase, encoded by Moloney Murine Leukemia Virus (M-MuLV RT) and expressed in E.coli is an RNA-dependent DNA polymerase lacking the RNase H activity. The enzyme synthesizes the cDNA first strand from a single-stranded RNA template to which a primer has been hybridized. M-MuLV RT will also extend primers hybridized to single-stranded DNA!
The enzyme is recommended for synthesis of cDNA from 100 bp up to 10 kb length.
Second strand cDNA synthesis can be achieved from some mRNA templates without an additional DNA polymerase.
Genaxxon offers the HotScriptase master mixes (M3062 >; M3064 >) with which it is possible to synthesize double stranded DNA completely without an isothermal transcription step. Time saving: up to 50%! No need for Superscript!
Money saving: high!
With our high quality dNTPs as Set (M3015.4100 and M3015.0250) or Mix (M3016.1010) or our DNA Ladders and our favourable standard agarose (M3044) we can offer additional high quality products for your PCR.
Documents:
ProtocolsManuals
Source: NCBI PubMed
RT-PCR: Eine Geschichte der Entfaltung der Genregulation
One step and Two step RT-PCR
RT-qPCR analysis of non-protein-coding RNA biomarker expression
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