One-Step RT-qPCR 2X Mastermix - Probe
Advantages at a glance
- Ultra-Fast: RT-qPCR in <35 minutes
- One-step RT-qPCR - One tube format
- For probe-based assays such as Taqman®, Beacons, MGB or Mediator Probes
- No need for Superscript!
- Money saving: high!
Also available with ROX!
Just ask us!
info@genaxxon.com
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.
Shipment: on wet ice. Store at -20°C. For laboratory usage only!
Our One-Step RT-qPCR 2X master mix was developed for quantitative real-time analyzes of RNA templates using probe-based assays such as Taqman®, Beacons, MGB or Mediator Probes and can be directly used with RNA as a starting material.
The ready-to-use mixture is based on a genetically modified reverse transcriptase with improved thermal stability, which offers increased specificity, a high cDNA yield and improved efficiency for highly structured and long cDNA fragments.
The 2X Mastermix contains all the reagents required for the RT-qPCR (except for the template, primer and probes) to ensure quick and easy handling at a minimum of pipetting steps. The high-quality enzymes and the optimized reaction buffer with high-purity dNTPs guarantee superior real-time PCR results. The sophisticated buffer system provides fast kinetics, a RNA sensitivity of <10 fg and target amplification even for difficult templates and multiplexing applications for more than 6 targets.
This Master Mix does not contain ROX - please make sure to choose the right setting on your qPCR cycler. A list of common thermal cyclers and their ROX compatibility can be found under the "Tips" tab.
RT-qPCR is used to amplify double-stranded DNA from single-stranded RNA templates to enable rapid real-time quantification of RNA targets. In the reverse transcription step, the reverse transcriptase synthesizes single-stranded DNA molecules (cDNA). The cDNA molecules are amplified by the HotStart DNA polymerase. The HotStart polymerase activity is blocked at ambient temperature and will be activated at the beginning of the initial denaturation. This thermal activation prevents the extension of non-specifically annealed primers and primer-dimer formations at low temperatures during the PCR setup.
One-Step RT-qPCR offers enormous convenience when analyzing targets from multiple RNA samples and minimizes the risk of contamination.
For RNA with a high degree of secondary structures or for complementary primers, the HotScriptase Master Mix (M3064) is particularly suitable. It enables the direct synthesis of double-stranded DNA without the time-consuming isothermal transcription step. Time savings: up to 50%.
With our high-quality dNTPs, available as a set (M3015) or as a mix (M3016), as well as our DNA markers and affordable standard agarose, we offer you additional products for PCR.
Time saving: up to 50%!
No need for Superscript!
Money saving: high!
Specifications:
Ready-to-use 2-time RT-qPCR mix
with Reverse Transcriptase
with Hot Start Polymerase
with RNase Inhibitor
with dNTPs
without ROX®
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-05-02
Documents:
ManualsCategory List
Source: NCBI PubMed
| Supplier | qPCR Cycler | ROX? |
| Applied Biosystems /Thermo Fischer | StepOne™ Real-Time PCR System | Yes (high ROX) |
| StepOnePlus™ Real-Time PCR System | Yes (high ROX) | |
| 7500 Real-Time PCR System | Yes | |
| 7500 Fast Real-Time PCR System | Yes | |
| 7500 Fast Dx Real-Time PCR Instrument | Yes | |
| 7500 Real-Time PCR System for Human Identification | Yes | |
| 7300 Real-Time PCR System | Yes (high ROX) | |
| Viia™ 7 Real-Time PCR System | Yes | |
| 7900HT Fast Real-Time PCR System | Yes (high ROX) | |
| OpenArray® Real-Time PCR Platform | Yes (high ROX) | |
| PRISM® 7000 Sequencing Detection System | Yes (high ROX) | |
| PRISM® 7700 Sequencing Detection System | Yes (high ROX) | |
| PRISM® 7900 Sequencing Detection System | Yes (high ROX) | |
| Gene Amp 5700 | Yes (high ROX) | |
| Quantstudio™ 3 / 3 FAST | Yes | |
| Quantstudio™ 5 / 5 FAST | Yes | |
| Quantstudio™ 6 | Yes | |
| Quantstudio™ 7 | Yes | |
| Quantstudio™ 12 | Yes | |
| QuantStudio™ 12K Flex system | Yes | |
| Bioneer | Exicycler™96 | No (optional) |
| Exicycler™384 | No (optional) | |
| Biorad | CFX96™ Real-Time PCR Detection System | No |
| CFX96 Touch™ Real-Time PCR Detection System | No | |
| CFX384 Touch™ Real-Time PCR Detection System | No | |
| CFX Opus 96 Real-Time PCR System | No | |
| CFX Opus 384 Real-Time PCR System | No | |
| CFX Connect™ Real-Time PCR Detection System | No | |
| iQ5 Real-Time PCR Detection System | No | |
| MiniOpticon™ Real-Time PCR Detection System | No | |
| Opticon 2 – Continuous Fluorescence Detection System | No | |
| Chromo4™ Four-Color Real-Time Detector | No | |
| DNA Technology | Dtlite | No (optional) |
| Dtprime | No (optional) | |
| Eppendorf | Mastercycler® ep realplex 4, Real-Time Thermal Cycler | No |
| Mastercycler® ep realplex 4s, Real-Time Thermal Cycler | No | |
| Mastercycler® ep realplex, Real-Time Thermal Cycler | No | |
| Mastercycler® ep realplex s, Real-Time Thermal Cycler | No | |
| Mastercycler Nexus | No | |
| Mastercycler Nexus gradient | No | |
| Mastercycler Pro | No | |
| Mastercycler Pro S | No | |
| Mastercycler Pro 384 | No | |
| Roche | LightCycler® 96 System | No |
| LightCycler® 480 System | No | |
| LightCycler® 2.0 Instrument | No | |
| LightCycler® 1.5 Instrument | No | |
| LightCycler® 1536 System | No | |
| LightCycler® Nano System | No | |
| Analytik Jena | qTower | Yes |
| qTower 2.0 | Yes | |
| qTower 2.2 | Yes | |
| qTower 3 | Yes | |
| qTower 3 84 | Yes | |
| Stratagene / Agilent | AriaMx Real-time PCR System | No |
| Mx3000P® qPCR System | Yes | |
| Mx3005P® qPCR System | Yes | |
| Mx4000® qPCR System | Yes | |
| Qiagen | Rotor-Gene™ Q | Yes |
| Rotor-Gene™ 6000 | Yes | |
| Quantabio | Q | No (optional) |
