RNase Inhibitor - Murine - recombinant
Advantages at a glance
- Glycerol free - lyo-ready
- 200 units/µL
- Inhibits common RNases (RNase A, RNase B, RNase C and human placental RNase)
- Does not inhibit RNase 1, RNase T1, RNase T2, S1 nuclease, and RNase H.
- Compatible with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases.
- Tested for the absence of DNases and RNases
Product number:
M3169.2000M
Delivery time 14 days
Shipment: on wet ice. Store at -20°C. For laboratory usage only!
Delivery time 14 days
Shipment: on wet ice. Store at -20°C. For laboratory usage only!
Delivery time
14 days
Product information "RNase Inhibitor - Murine - recombinant"
The Genaxxon RNase Inhibitor is a recombinant (E.coli) murine protein which inhibits ribonucleases (RNases) A, B and C. It does not inhibit RNase 1, RNase H, RNase T1, S1 Nuclease. Native RNase-Inhibitor from murine placenta exerts its inhibitory effect by binding non-covalently to RNases in a 1:1 ratio with high affinity.
There is no inhibition of polymerase activity when this RNase Inhibitor is used with AMV Reverse Transcriptase, M-MuLV Reverse Transcriptase, Taq DNA Polymerase or Phage RNA Polymerase (SP6, T7, or T3).
The enzyme is active over a broad pH range between 5 and 9, with a maximum activity at pH7 – pH8.
The inhibition of RNases is a reversible process. Reagents as urea or thiol reagents will lead to the dissociation of the Inhibitor-RNase-complex, resulting in re-natured and functional RNase and irreversibly deactivated RNase inhibitor.
Applications:
- RT-PCR
- cDNA synthesis
- In vitro transcription/translation
- Enzymatic RNA labelling reactions
- In vitro virus detection.
- Improvement of RNA translation in homologous systems.
- Preparations of RNase-free antibodies.
- Any application where eukaryotic RNase contamination is a potential problem.
Advantages:
The recombinant murine RNase inhibitor does not contain the cysteine pair that was identified to cause the high sensitivity against oxidation resulting in the inactivation of the human RNase inhibitor (1). As a result, the murine RNase inhibitor has significantly improved oxidation resistance compared to the human and porcine RNase inhibitors. In addition, murine RNase inhibitor is much more stable at low low DTT concentrations (less than 1 mM), what makes it ideal for reactions where high DTT concentrations interfere with the reaction (e.g. real-time RT-PCR).
Product does not contain glycerol => lyo-ready product (ready for preparations of freeze-dried products).
Recommendations:
Since the RNase inhibitor protein is sensitive to denaturing agents and conditions, whereas RNases are not, care must be taken to avoid high concentrations of urea or other denaturing agents and temperatures above 50-55°C.
The recommended concentration of the RNase inhibitor is 1 unit/µL.
During set-up of a reaction, RNase inhibitor should be added before other components are added (enzymes, plasmids from mini preps, etc.).
References
1. Kim, B.M. et al. Protein Science. 8, 430-434.
200 units/µL
Recombinant protein of murine origin
Inhibits common eukaryotic RNases (RNase A, RNase B, RNase C and human placental RNase)
Does not inhibit RNase 1, RNase T1, RNase T2, S1 nuclease, and RNase H.
Compatible with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases.
Active over a broad pH range (pH 5-8).
Tested for the absence of DNases and RNases
Applikation / Application:
- Reversible inhibition of RNase- Suitable for all applications with RNA, especially for reverse transcription of RNA into cDNA- Useful if eukaryotic RNase contamination occurs
Einheiten / Units:
one unit of RNase inhibitor is defined as the amount of protein required to inhibit 50% of the activity of 5ng of RNase A
Quelle / Source:
E. coli strain that carries the murine Ribonuclease Inhibitor gene
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-04-10
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.
Documents:
Safety Data SheetProduct description
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Source: NCBI PubMed
Source: NCBI PubMed
