Scriptase RT - cDNA Synthesis kit
Advantages at a glance
- Complete Kit with Primers and DNase: Everything you need for successful reverse transcription.
- Suitable for Templates up to 17 kb: Perfect for large RNA samples.
- High Yield: Reduced RNase-H activity ensures optimal results.
- Fast Process: Complete reverse transcription in under 10 minutes.
- Flexible Primer Options: Use specific primers, Random Hexamers, or Oligo(dT) primers
Product number:
SM3134.0500
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.
Shipment: on wet ice. Store at -20°C. For laboratory usage only!
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.
Shipment: on wet ice. Store at -20°C. For laboratory usage only!
Product information "Scriptase RT - cDNA Synthesis kit"
Genaxxon bioscience Scriptase RT is a proprietary reverse transcriptase with reduced RNase-H activity and increased thermostability that guarantees high specificity, longer cDNAs and a higher yield even with GC-rich target RNAs. With the included Oligo(dT) and Random Hexamer Primer you can easily optimise the protocol according to your needs.
We recommend Scriptase RT for the synthesis of cDNA from 100 bp up to 10 kb in length.
Properties
- Ready-to-use kit incl. primer and DNase
- Suitable for templates up to 17 kb
- High yield due to reduced RNase-H activity
- Complete reverse transcription in less than 15 minutes
- For specific primers, random hexamers or oligo(dT) primers
- reverse Transcription up to 65°C possible for complex transcripts
Advantages
- High sensitivity
- cDNA in <15 minutes
- Primers included
- gDNA removal
- Temperature stability
Applications
Fast and easy cDNA synthesis with just one kit. The Scriptase RT kit offers high sensitivity and specificity. It is also very flexible and delivers high yields with both oligo(dT) and random hexamers or gene-specific primers.
Gene expression levels in tissues or different cell types can be determined by the detection of specific mRNA. For this purpose, the total RNA is first transcribed into cDNA. In the subsequent qPCR with gene-specific primers, the respective expression level is determined.
In the diagnosis of RNA viruses, reverse transcription is performed upstream of sequencing in order to create a cDNA library. In addition to pathogen detection itself, it can also be used to create mutation profiles of pathogens.
As part of an RNA-seq workflow, mRNA enrichment can be performed using reverse transcription in combination with oligo(dT) primers. This allows both mRNA selection and RT to be performed in one step, which is particularly useful for small amounts of RNA. It should be noted that a 3′ bias may occur during subsequent sequencing. This would result in an increased number of reads for the 3′ end of the RNAs.
FAQs
No search results.
For most assays, we recommend either using a 1:1 mixture of Random Hexamer and OligoDT(20) primers or using Random Hexamer primers alone.
No, you do not need to. The RNase inhibitor included in the kit prevents RNA degradation, so there’s no need to pipette on ice.
The Scriptase RT-cDNA Synthesis Kit allows for cDNA synthesis even from samples with very low RNA amounts (1 pg - 5 µg).
Removing genomic DNA is recommended because even primers that do not bind to genomic DNA can produce a signal in qPCR. Therefore, the Scriptase RT-cDNA Synthesis Kit includes a DNase enzyme mix for genomic DNA removal.
Comes with:
Scriptase RT: 200 units/µL in storage buffer containing 50% glycerol (v/v).
5X RT Reaction Buffer: 250mM Tris/HCl (pH8.3), 375mM KCl, 15mM MgCl2, 50mM DTT
dNTP-Mix 10 mM each dNTP (dATP, dCTP, dGTP, dTTP)
Oligo-(dT)20 primer 100µM
Random Hexamers 100µM
RNase Inhibitor 40 units/μL. RNase inhibitor in storage buffer with 50% glycerol (v/v)
10X DNase buffer 40 units/µL.
DNase enzyme mix 5 units/µL
RNase-free water
Applikation / Application:
cDNA synthesis
Einheiten / Units:
Quelle / Source:
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-16-05-02
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.
Documents:
Safety Data SheetProduct description
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Source: NCBI PubMed
Source: NCBI PubMed