Real-time PCR, also known as qPCR, is undoubtedly an impressive method for measuring the amplification of target DNA in real time. However, some people may despair at the abundance of terms, protocols and kits on the market. This begs the question: are reliable qPCR results only achievable through magic or is it ultimately more about the art of precise fine-tuning? We can reassure you: in fact, with just a few tricks you can optimize your qPCR so that you can easily achieve reliable and accurate results. Whether you are already an expert in the field of qPCR or just taking your first steps into this fascinating world, this article will guide you through the key factors that will ensure your qPCR assays produce the best possible results. From deciding on the method, to quality control, to optimizing specificity - we have important tips and suggestions for you.

The world of genomics and molecular biology has taken a breathtaking leap into the future in recent years. In addition to "high fidelity" enzymes, "highly discriminating" enzymes such as the highly selective SNP DNA polymerases from Genaxxon have become the superstars of the laboratory. These enzymes are specifically modified for highly selective PCR tests and mutation detection - they help you when you want to find "the needle in the haystack".

Since the isolation of the first Taq DNA polymerase in 1976, PCR has become an integral part of the molecular biology laboratory. Of course, methods, reagents and protocols have evolved. The goal is clear: to make it better, faster, cheaper, and easier. Every day, lab technicians, students and scientists around the world spend countless hours pipetting and preparing PCR experiments. This makes it even more frustrating when errors and contamination only become apparent in the final step of the PCR workflow. But how can the work in the PCR laboratory be optimised to ultimately minimise sources of error and at the same time save time, money, and nerves? We at Genaxxon would like to shed some light into the darkness of the laboratory and show how our new products around the Red Mastermix Fluoro (2X) can help to optimise your PCR workflow along a typical PCR workflow.

Progress in the field of SNP genotyping and its applications, from the perspective of plant breeding and agriculture

Sanger sequencing technology was the first generation of sequencing and helped provide basic knowledge about genome sequencing but didn’t have the potential for its further improvement. Hence a new technological jump in sequencing expertise was required. That’s when Next Generation Sequencing techniques came into existence and revolutionized our entire capability of genome sequencing.

In the articles below you will find the authors already relying on the Genaxxon products. We say thank you to these authors, trusting in our service. Let us convince you as well.

Wine production, while considered an art form, owes an increasing debt to the latest molecular genetic technologies. Not only do these facilitate batch-to-batch consistency, they also enable the generation of novel strains of grape microflora. While the choice of grapes is important, so too is the interplay between extrinsic and intrinsic factors, and the technological decisions made at every step in the process. Together, these determine whether the finished product will be classed as a “vintage wine”, a “quaffing wine”, or “vinegar”.

Nowadays there are many different suppliers and systems for polymerase chain reaction (PCR) and of its derivative techniques, quantitative, or real-time, PCR (qPCR) and reverse transcription PCR (RT-PCR). Competition gives you the opportunity to simplify diagnostics and to make research more financially favorable.

There's no accounting for taste. Cilantro (Coriander): some people love it in their food, some hate it. Here we are detecting a genomic SNP (rs72921001) in HeLa genomic DNA. This SNP is reported to be close to a number of genes coding for olfactory receptors. (Reference: Eriksson N. et al. (2012), "A genetic variant near olfactory receptor genes influences cilantro preference.") Considering, that only the C-allele specific primer is extended and yielding in a specific amplicon, we can conclude a genetic predisposition in disliking cilantro, as this SNP is significantly associated with detecting a soapy taste to cilantro.

Welcome to GENAXXON bioscience. We Aim for Your Success. For more than 20 years, we have been offering our clients a broad range of high quality PCR and qPCR products. Our goal is to serve our custome...

Listed below are articles and references, in which the authors trust in the high quality of this GENAXXON Product. Source: PubMed NCBI > Immunomodulatory role of reactive oxygen species and nitroge...