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GCSF is a cytokine that controls the production, differentiation, and function of granulocytes. The active protein is found extracellularly. Three transcript variants encoding three different isoforms have been found for the GCSF gene. Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This csf induces granulocytes.
Rec. human Granulocyte Colony Stimulating Factor produced in E.coli is a single, non-glycosylated, polypeptide chain of 175 amino acids and a molecular mass of 18.8 KDa.
Biological activity: The ED50, calculated by the dose-dependant proliferation of murine NFS-60 indicator cells (measured by 3H-thymidine uptake) is <0.1ng/mL, corresponding to a Specific Activity of 100,000,000 IU/mg.
Synonyms: CSF-3, MGI-1G, GM-CSF beta, Pluripoietin, Filgrastim, Lenograstim, G-CSF, MGC45931, GCSF.
Purity: >98% (HPLC, SDS-PAGE)
GCSF was lyophilized after extensive dialysis against 10mM sodium acetate buffer pH=4.
First five amino acids: Met-Thr-Pro-Leu-Gly.
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 34-16-04-90
Dokumente - Protokolle - Downloads
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