10X Phosphate buffered saline powder (pH7.4) - 5 bags

Genaxxon bioscience

Advantages at a glance

Pre-mixed buffer powder with pre-set pH
Eliminates calculation, formulation and weighing errors
Dissolve-and-go for greater convenience
Stable at room temperature for at least 3 years
Guaranteed reproducibility

€107.12*

Prices excl. VAT plus shipping costs

pack size

5 bag

Product number: D2016.1005
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.

Shipment: not cooled. Store at +15°C to +30°C. For laboratory usage only!

Product information "10X Phosphate buffered saline powder (pH7.4) - 5 bags"

Among biological buffers PBS is one of the most commonly used. The buffer is isotonic and non-toxic to cells and has the ability to maintain their osmolarity. Thereby the buffer is suitable for washing procedures in cell cultures and for immunoassays such as ELISA and immuno-histochemical procedures. It is often used for sample dilution in molecular biology and as protein diluent in Western blotting. Furthermore, the buffer can function as an equilibrator for gel filter columns.

This PBS buffer is specifically developed for immunological and microbiological laboratories. It is provided as pre-weighed tablets in containers and in convenient blister packs, or as pre-weighed powder mix in sealed pouches.

How to prepare buffer solution:

Empty one pouch of NaPi buffer in a laboratory flask or beaker placed on a magnetic stirrer. Add 200mL to 300mL of deionized water for a 1000mL pouch and stir the solution for a few minutes. Adjust the volume up to 1000mL, stir and the buffer solution is ready to use.

Other phosphate-based ready-to-use buffers::

PBS without Potassium (0.01M) (D2033 >).
PBS with Tween™20, pH7.4 (D2003 (100mL and 500mL) > and (D2004 (1L) >).
PBS, pH7.2 (D2034 >)

PBS with Potassium, pH7.4 (D2000 - 100mL >; D2001 - 500mL >; D2002 - 1L >; D2016 - powder, 0,1M, 1L >; D2017 - powder, 0,01M, 10L bis 100L >).

Here you will find all our ready-to-use, pre-mixed buffers as powder or tablets >

Specifications:
Purity of used chemicals: min. 99.0%.

Content of 1 pouch dissolved in deionized water and made up to 1000mL yields: 0.10M phosphate buffer, 0.027M potassium chloride, 1.40M sodium chloride, pH7.4 at 25°C (pH of 1-time concentration).

Applikation / Application:

- Wash buffer in immunolabelling techniques, such as ELISA and Western blotting - Blocking buffer for plate based assays - Protein-plate coating

Einheiten / Units:

Quelle / Source:

synthetic

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 32-16-04-90

Documents - Protocols - Downloads :

Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.

Documents:

Safety Data Sheet
Certificate
General Data 1
General Data 2
Category List

Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Source: NCBI PubMed