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Among biological buffers PBS is one of the most commonly used. The buffer is isotonic and non-toxic to cells and has the ability to maintain their osmolarity. Thereby the buffer is suitable for washing procedures in cell cultures and for immunoassays such as ELISA and immuno-histochemical procedures. It is often used for sample dilution in molecular biology and as protein diluent in Western blotting. Furthermore, the buffer can function as an equilibrator for gel filter columns.
This PBS buffer is specifically developed for immunological and microbiological laboratories. It is provided as pre-weighed tablets in containers and in convenient blister packs, or as pre-weighed powder mix in sealed pouches.
There are seventeen standard-sized packages and final volumes range from 100mL to 100 litres. Choose from different pH, 7.2 and 7.4 and from PBS with or without potassium. PBS without potassium is available in two different molar strengths, 0.01M and 0.02M.
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How to prepare buffer solution:
Empty one pouch of NaPi buffer in a laboratory flask or beaker placed on a magnetic stirrer. Add 200mL to 300mL of deionized water for a 1000mL pouch and stir the solution for a few minutes. Adjust the volume up to 1000mL, stir and the buffer solution is ready to use.
pre-weight ready-to-use certified
Purity of used chemicals: min. 99.0%.
Content of 1 pouch dissolved in deionized water and made up to 1000mL yields: 0.10M phosphate buffer, 0.027M potassium chloride, 1.40M sodium chloride, pH7.4 at 25°C (pH of 1-time concentration).
application:- Wash buffer in immunolabelling techniques, such as ELISA and Western blotting - Blocking buffer for plate based assays - Protein-plate coating
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 32-16-04-05
Dokumente - Protokolle - Downloads
Here you will find information and further literature on 10X Phosphate buffered saline powder (pH7.4) - 5 bags. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: firstname.lastname@example.org or phone: +49 731 3608 123.