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TBS Tween® 20 (Tris buffered saline with Tween) is one of the most commonly used biological buffers for plate based immunoassays and Western blotting. It is isotonic, non-toxic to cells and contains a non-ionic detergent. It is used preferably as an antibody diluent and wash reagent in ELISA and Western blotting using alkaline phophatase or peroxidase-conjugated antibodies. TBS-T buffer is supplied in exactly pre-weighed tablets, each tablet giving 500mL of 0.15M NaCl, 0.05% Tween® 20, 0.050M Tris-HCl buffer solution with pH7.6 at 25°C.
Directions for use: Deposit one tablet in a laboratory flask or beaker placed on a magnetic stirrer. Add 200mL of deionized water and stir the solution for a few minutes. Adjust the volume up to 500mL, stir until full dissolution and the buffer is ready to use.
pre-weight ready-to-use certified
Exactly pre-weight powder mix.
Concentrations if dissolved in 500mL water: 0.05M Tris/HCl buffer, 0,15M sodium chloride, 0.05% Tween® 20, pH7.6 +/-0.1 (25°C).
All ingredients used for the buffer powder are of highest purity.
application:Immuno-Assays Westernblotting ELISA
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 32-16-04-05
Dokumente - Protokolle - Downloads
Here you will find information and further literature on TBS with Tween®20, pH7.6 (500mL). For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: email@example.com or phone: +49 731 3608 123.