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IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used together with X-Gal > (5-bromo-4-chloro-3-indolyl-ß-D-galactoside) or Bluo-Gal > (5-Bromo-3-indolyl-beta-D-galactopyranoside) in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli.
Mode of operation:
While IPTG binds to the lacI repressor it is altering the conformation of the lacl repressor, which prevents the repression of the β-galactosidase coding gene lacZ. Thus IPTG is an inducer of the lac operon in bacteria for detection of lac+ colonies.
Dissolve in water to e.g. 200mg/mL, sterilise by filtration and store in aliquots at -20°C. For detection of transformants, use in final concentration of 0.1mM up to 1mM in LB-Medium.
Assay (HPLC): min. 99.5%
MP = 110-112°C
Specific optical rotation (a20°/D, c=1 water): -31°+/-2°C
Water: max. 0.5%
Dioxane: not detectable
MW = 238.3 g/mol
Sicherheits Hinweise / Safety
Klassifizierungen / ClassificationEC-Nr: 206-703-0
Dokumente - Protokolle - Downloads
Here you will find information and further literature on HighPure IPTG, dioxane free, (min. 99.5%). For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: firstname.lastname@example.org or phone: +49 731 3608 123.