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IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used together with X-Gal > (5-bromo-4-chloro-3-indolyl-ß-D-galactoside) or Bluo-Gal > (5-Bromo-3-indolyl-beta-D-galactopyranoside) in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli.
Mode of operation:
While IPTG binds to the lacI repressor it is altering the conformation of the lacl repressor, which prevents the repression of the β-galactosidase coding gene lacZ. Thus IPTG is an inducer of the lac operon in bacteria for detection of lac+ colonies.
Dissolve in water to e.g. 200mg/mL, sterilise by filtration and store in aliquots at -20°C. For detection of transformants, use in final concentration of 0.1mM up to 1mM in LB-Medium.
Purity: min. 99% (HPLC)
Appearance: white powder
RNases, DNases: not detected
MP = 110-112°C
Specific rotation (a20°/D, c=1 water): -28.5° - -34.5°
Water: max. 1%
Dioxane: 1.4-Dioxane not detected
Inhibitor free: tested active in Lac-gene expression
MW = 238.3 g/mol;
application:IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli.
Sicherheits Hinweise / Safety
Klassifizierungen / ClassificationEC-Nr: 206-703-0
Dokumente - Protokolle - Downloads
Here you will find information and further literature on IPTG, dioxane free, Molbio Grade. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: email@example.com or phone: +49 731 3608 123.