Order number: M3198.0010Shipping: shipped at RT, stored at +2°C to +8°C
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IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used together with X-Gal > (5-bromo-4-chloro-3-indolyl-ß-D-galactoside) or Bluo-Gal > (5-Bromo-3-indolyl-beta-D-galactopyranoside) in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli.
Mode of operation:
While IPTG binds to the lacI repressor it is altering the conformation of the lacl repressor, which prevents the repression of the β-galactosidase coding gene lacZ. Thus IPTG is an inducer of the lac operon in bacteria for detection of lac+ colonies.
Dissolve in water to e.g. 200mg/mL, sterilise by filtration and store in aliquots at -20°C. For detection of transformants, use in final concentration of 0.1mM up to 1mM in LB-Medium.
Purity: min. 99% (HPLC)
Appearance: white powder
RNases, DNases: not detected
MP = 110-112°C
Specific rotation (a20°/D, c=1 water): -28.5° - -34.5°
Water: max. 1%
Dioxane: 1.4-Dioxane not detected
Inhibitor free: tested active in Lac-gene expression
MW = 238.3 g/mol;
application:IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli.
Sicherheits Hinweise / Safety
Klassifizierungen / ClassificationEC-Nr: 206-703-0