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IPTG, dioxane free, Molbio Grade

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  • High-quality products
  • Customized solutions
  • Personal contact
  • Fast service
  • Competent technical support +49(0)731-3608-123

Quantity Unit price
To 1
€280.00*
To 3
€238.00*
€280.00* (15% saved)
To 7
€196.00*
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From 8
€154.00*
€280.00* (45% saved)
Weight
Product number: M3198.0025
Ready to ship today,
Delivery time 1-2 workdays

Shipment: not cooled. Store at +2°C to +8°C. Protect from light. For laboratory usage only!
Product information "IPTG, dioxane free, Molbio Grade"

IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used together with X-Gal > (5-bromo-4-chloro-3-indolyl-ß-D-galactoside) or Bluo-Gal > (5-Bromo-3-indolyl-beta-D-galactopyranoside) in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli.

Mode of operation:
While IPTG binds to the lacI repressor it is altering the conformation of the lacl repressor, which prevents the repression of the β-galactosidase coding gene lacZ. Thus IPTG is an inducer of the lac operon in bacteria for detection of lac+ colonies.

Dissolve in water to e.g. 200mg/mL, sterilise by filtration and store in aliquots at -20°C. For detection of transformants, use in final concentration of 0.1mM up to 1mM in LB-Medium.

Specifications:
Purity: min. 99% (HPLC)
Appearance: white powder
RNases, DNases: not detected
MP = 110-112°C
Specific rotation (a20°/D, c=1 water): -20.0° - -34.0°
Water: max. 0.5%
Dioxane: 1.4-Dioxane not detected
Inhibitor free: tested active in Lac-gene expression
C9H18O5S
MW = 238.3 g/mol


Applikation / Application:
IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli.

Einheiten / Units:
Quelle / Source:
synthetic


Sicherheits Hinweise / Safety


Klassifizierungen / Classification

EC-Nr: 206-703-0
CAS-Nr: 367-93-1
eclass-Nr: 32-16-04-90
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.


Documents:

Safety Data Sheet
Certificate
Category List
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Source: NCBI PubMed