Salmon Sperm DNA (Na salt)
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Delivery time: 3- 8 working days
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Shipment: not cooled. Store at +2°C to +8°C. For laboratory usage only!
DNA extracted from salmon testes. To prevent unspecific binding of a DNA to the membrane in Southern- and Northern-Hybridisations salmon sperm DNA is added to the pre-hybridisation solution (Blotto, Denhardt's etc.). This Salmon Sperm DNA shows a molecular weight of ca. 10 - 2500 bp (20 - 650000 Da) with the main component of approx. 2000 bp.
The lyophilised DNA can be dissolved in sterile bidistilled water (stock solution e. g. 10mg/mL) and stored at -20°C. This DNA is used with a final concentration of 100μg/mL (Ref.: Sambrook, J., et al., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, NY (1989), 9.48-9.49, 13.15. Silhavy, T., et al., Experiments with Gene Fusions, Cold Spring Harbor, NY (1984), 195.)
Specifications:
Low molecular weight DNA
DNAse and RNAse activities: not detected
protein free
very low bioburden
MW: <2500 Da (main component: approx. 2000 bp)
lyophilised powder
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
EC-Nr: 309-566-6CAS-Nr: 9007-49-2
eclass-Nr: 32-16-04-14
Documents:
CertificateSource: NCBI PubMed
