Amino Acid Analysis

Need exact information about the purity, composition, or the molecular weight of your protein? You want to know the exact amount of free or bound amino acids in your sample (classical protein/peptide sample, seed, blood, serum, urine or other body materials) or the amount of peptide coupled to a solid matrix?

We can offer this information. Contact us and inquire about the exact procedure. Once all the details are clear, you can send us your samples for rapid and reliable examination by quantitative amino acid analysis (ASA). e-mail:info@genaxxon.com

After receiving the sample we will send you an official order confirmation with expected date of delivery of the analytical results by e-mail.

Our service includes:

sample hydrolysis: The hydrolysis is for cleavage of all peptide bonds in a protein/peptide to enable chromatographic separation and quantification of the free amino acids. The proteins/peptides to be analyzed can be derived from living or dead organisms from food and feed (dairy, meat and sausages, beer, drinks, plants), seed or from blood, serum and urine.

post-column derivatization using ninhydrin: To be able to detect and quantify the released amino acids, these are chemically modified by a fluorescent dye or by ninhydrin to enable detection and quantification by their absorption signal and signal intensity in an UV-chromatogram.

evaluation and transmission of the analysis results: From the obtained raw data we provide a meaningful analysis report together with all important information on the composition of the protein/peptide or the sample matrix. These data may be as follows: sample concentration in nmol/mL, in mg/mL or in nmol/mg matrix. In addition, you will get an overview of the number of each individual amino acid.

The analysis report is sent as a data file (pdf) by e-mail, including the respective raw data.