Amino Acid Analysis of given Sample (total hydrolysis)

Amino Acid Analysis of given Sample (total hydrolysis)


Order number: P2145.0001

Delivery time 14 Workdays

€172.24 *

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The amino acid analysis service encloses the total hydrolyis of a given sample, the separation... more
Product information "Amino Acid Analysis of given Sample (total hydrolysis)"

The amino acid analysis service encloses the total hydrolyis of a given sample, the separation of the amino acids by reversed phase HPLC, the chemical derivatisation of the free amino acids with Ninhydrin (either produced by the acid hydrolysis or of already free amino acids), and transfer of a comprehensive analysis report as pdf-file.

Pure samples are required. The presence of salts, buffers, or detergents are deleterious. Amines (primary or secondary) will react with the carbamate, adversely affecting results. While salts, especially strong buffer substances can alter the pH of the sample causing the hydrolysis/derivitisation to be incomplete or simply fail. Additionally, significant levels of glycerol or carbohydrates are problematic - the glycerol is nonvolatile and attracts moisture (acid) and carbohydrates char, decomposing to ash taking the sample with them. Samples containing Chitin or samples from bones, cartilage or blood plasma can be normally analysed without problems.

For analysis of proteins we do need 25µg to 100µg (500pmol to 2nmol) based on the molecular weight of each protein. For peptides, the requirement is much smaller - 5µg to 20µg (5nmol to 25nmol) will be ok.

The total hydrolysis (P2145.0001 >) is necessary when the analysis of bound amino acids is conducted. No total hydrolysis is required for the analysis of free amino acids (P2145.0002 >). As Methionin and Cysteine are destroyed during total hydrolysis, we offer the oxidative total hydrolysis for quantification of these amino acids (P2145.0005 >).

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Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 25-14-10-90
Dokumente - Protokolle - Downloads more

Dokumente - Protokolle - Downloads

Here you will find information and further literature on Amino Acid Analysis of given Sample (total hydrolysis). For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.

 
 
 
Literatur .... more

Referenzen..

Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.

Quelle/Source: NCBI PubMed >

Impact of spatial organization on a novel auxotrophic interaction among soil microbes

Xue Jiang, Christian Zerfaß, Song Feng, Ruth Eichmann, Munehiro Asally, Patrick Schäfer, Orkun S Soyer

ISME J. 2018 Jun; 12(6): 1443–1456. Published online 2018 Mar 23. doi: 10.1038/s41396-018-0095-z

PMCID: PMC5955953

 

The giant keyhole limpet radular teeth: A naturally-grown harvest machine

Tina Ukmar-Godec, Gregor Kapun, Paul Zaslansky, Damien Faivre

J Struct Biol. 2015 Dec; 192(3): 392–402.  doi: 10.1016/j.jsb.2015.09.021

PMCID: PMC4658332

 

The plastid outer envelope protein OEP16 affects metabolic fluxes during ABA-controlled seed development and germination

Birgit Pudelski, Annette Schock, Stefan Hoth, Ruslana Radchuk, Hans Weber, Jörg Hofmann, Uwe Sonnewald, Jürgen Soll, Katrin Philippar

J Exp Bot. 2012 Mar; 63(5): 1919–1936.  Published online 2011 Dec 9. doi: 10.1093/jxb/err375

PMCID: PMC3295387

 

A Protein Epitope Signature Tag (PrEST) Library Allows SILAC-based Absolute Quantification and Multiplexed Determination of Protein Copy Numbers in Cell Lines

Marlis Zeiler, Werner L. Straube, Emma Lundberg, Mathias Uhlen, Matthias Mann

Mol Cell Proteomics. 2012 Mar; 11(3): O111.009613.  Published online 2011 Sep 30. doi: 10.1074/mcp.O111.009613

PMCID: PMC3316735

 

Gene Cluster Involved in the Biosynthesis of Griseobactin, a Catechol-Peptide Siderophore of Streptomyces sp. ATCC 700974

Silke I. Patzer, Volkmar Braun

J Bacteriol. 2010 Jan; 192(2): 426–435.  Published online 2009 Nov 13. doi: 10.1128/JB.01250-09

PMCID: PMC2805312

 

 
 
 
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