Order number: P2145.0001
Delivery time 14 Workdays
The amino acid analysis (total hydrolyis) of a given sample, the separation of the amino acids by reversed phase HPLC, the chemical derivatisation of the free amino acids with Ninhydrin (either produced by the acid hydrolysis or of already free amino acids), and transfer of a comprehensive analysis report as pdf and excel file. As acid hydrolysis will completely (or at least partially) destroy , the amino acids cysteine and methionine they can not be quantified by this method. In order to be able to quantify Cys and Met, an oxidation step with performic acid must be carried out. See P2145.0005 > "Amino acid analysis (oxidative hydrolysis).
Pure samples are required. The presence of salts, buffers, or detergents are deleterious. Amines (primary or secondary) will react with the carbamate, adversely affecting results. While salts, especially strong buffer substances can alter the pH of the sample causing the hydrolysis/derivitisation to be incomplete or simply fail. Additionally, significant levels of glycerol or carbohydrates are problematic - the glycerol is nonvolatile and attracts moisture (acid) and carbohydrates char, decomposing to ash taking the sample with them. Samples containing Chitin or samples from bones, cartilage or blood plasma can be normally analysed without problems.
For analysis of proteins we do need 25µg to 100µg (500pmol to 2nmol) based on the molecular weight of each protein. For peptides, the requirement is much smaller - 5µg to 20µg (5nmol to 25nmol) will be ok.
The total hydrolysis (P2145.0001 >) is necessary when the analysis of bound amino acids is conducted. No total hydrolysis is required for the analysis of free amino acids (P2145.0002 >). As Methionin and Cysteine are destroyed during total hydrolysis, we offer the oxidative total hydrolysis for quantification of these amino acids (P2145.0005 >).
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 25-14-10-90
Dokumente - Protokolle - Downloads
Here you will find information and further literature on Amino Acid Analysis of given Sample (total hydrolysis). For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: email@example.com or phone: +49 731 3608 123.
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