Amino Acid Analysis of given Sample (without total hydrolysis)

The amino acid analysis service encloses the separation of the amino acids by reversed phase HPLC, the chemical derivatisation of the free amino acids with Ninhydrin (either produced by the acid hydrolysis or of already free amino acids), and transfer of a comprehensive analysis report as pdf-file.

Pure samples are required. The presence of different salts, or detergents are deleterious. Amines (primary or secondary) will react with the carbamate, adversely affecting results. Additionally, significant levels of glycerol or carbohydrates are problematic - the glycerol is nonvolatile and attracts moisture (acid) and carbohydrates char, decomposing to ash taking the sample with them.

Samples containing Chitin or samples from bones, cartilage or blood plasma can be normally analysed without problems.

For analysis of proteins we do need 25µg to 100µg (500pmol to 2nmol) based on the molecular weight of each protein. For peptides, the requirement is much smaller - 5µg to 20µg (5nmol to 25nmol) will be ok.

The total hydrolysis (P2145.0001 >) is necessary when the analysis of bound amino acids is conducted. No total hydrolysis is required for the analysis of free amino acids (P2145.0002 >). As Methionin and Cysteine are destroyed during total hydrolysis, we offer the oxidative total hydrolysis for quantification of these amino acids (P2145.0005 >).