Order number: P2011.0010Shipping: shipped and stored on wet ice at -20°C
Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.
No target guide RNA for CRISPR is purified RNA, which has no genomic target in human, rat and mouse.
This non-targeting gRNA serves as a tool for establishing non-toxic transfection conditions, or as a negative control in down-stream assays, monitoring the success of a genome editing experiment.
Other Cas9-NLS proteins from Genaxxon: Cas9-NLS (nuclease, wildtype) >, Cas9-NLS-tagRFP (nuclease, with red fluorescent protein tag) >; Cas9-Nickase-NLS (nickase) >; Cas9-Dead-NLS (none) >
Transfection reagent for the co-transfection RNA/protein, specially designed for use in CRISPR/Cas9 systems: CRISPRfect E >.
negative control for transfection experiments
Concentration: The standard concentration is 400 ng/µL in water. Custom concentrations are possible.
Quality control: All gRNAs from Genaxxon are designed based on the algorithms by Doench et al. (2014, Nature Biotechnology 32: 1262-1267) and Moreno-Mateos et al. (2015, Nature Methods 12: 982-988). The quality of each synthesized gRNA is checked via gel electrophoresis, and the concentration is determined by measuring A260. Each gRNA must pass a cell toxicity test.
Sicherheits Hinweise / Safety
Klassifizierungen / ClassificationCAS-Nr: 39665-12-8
Dokumente - Protokolle - Downloads
Here you will find information and further literature on No target guide RNA for CRISPR experiments. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: email@example.com or phone: +49 731 3608 123.
Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
In vivo topology converts competition for cell-matrix adhesion into directional migration
Fernanda Bajanca, Nadège Gouignard, Charlotte Colle, Maddy Parsons, Roberto Mayor, Eric Theveneau
Nat Commun. 2019; 10: 1518. Published online 2019 Apr 3. doi: 10.1038/s41467-019-09548-5