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The Genaxxon Cas9-NLS represents the wild-type Cas9 nuclease from S. pyogenes, fused with a C-terminal NLS. Currently, the best-established Cas9 protein in the field with most publications. Cas9-NLS (CRISPR associated protein 9) is an RNA-guided DNA endonuclease enzyme associated with the CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) adaptive immunity system in Streptococcus pyogenes, among other bacteria. S. pyogenes utilizes Cas9 to remember and later probe and cleave foreign DNA, such as invading bacteriophage DNA or plasmid DNA. In the case that the DNA substrate is complementary to the guide RNA, Cas9 cleaves the invading DNA. In addition to its original role in bacterial immunity, the Cas9 protein has been heavily employed as a genome engineering tool to induce site-directed double strand breaks in DNA. These disruptions may lead to gene inactivation or the presentation of heterologous genes via non-homologous end joining and homologous recombination respectively in many laboratory model organisms.
Recombinant Cas9-NLS comprises the entire Cas9 protein sequence (1368 amino acids) joined to a 6xHis tag at the N-terminus and a proprietary nuclear localization sequence (NLS) at the C-terminus. The protein has an apparent molecular weight of 160kDa on SDS-PAGE.
Synonyms: CRISPR-associated endonuclease Cas9/Csn1, SpyCas9, cas9, csn1.
Other Cas9-NLS proteins from Genaxxon: Cas9-NLS (nuclease, wildtype) >, Cas9-NLS-tagRFP (nuclease, with red fluorescent protein tag) >; Cas9-Nickase-NLS (nickase) >; Cas9-Dead-NLS (none) >
Transfection reagent for the co-transfection RNA/protein, specially designed for use in CRISPR/Cas9 systems: CRISPRfect E >.
Specific genome targeting Labelling of target sites.
Purity: >97% (determined by SDS-PAGE).
Recombinant Cas9-NLS comprises the entire Cas9 protein sequence and the nuclear localization sequence PKKKRKV (= NLS of Simian virus 40) at the C-terminus. The protein has an apparent molecular weight of 160kDa on SDS-PAGE.
application:CRISPR Cas9 Cloning
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 34-16-04-90
Dokumente - Protokolle - Downloads
Here you will find information and further literature on Cas9-NLS (CRISPR associated protein 9). For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: firstname.lastname@example.org or phone: +49 731 3608 123.
Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
In vivo topology converts competition for cell-matrix adhesion into directional migration
Fernanda Bajanca, Nadège Gouignard, Charlotte Colle, Maddy Parsons, Roberto Mayor, Eric Theveneau
Nat Commun. 2019; 10: 1518. Published online 2019 Apr 3. doi: 10.1038/s41467-019-09548-5