Cas9-Dead-NLS-EGFP

Cas9-Dead-NLS-EGFP


Order number: S5316.0010

Shipping: shipped on wet ice, stored at -20°C

Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.

€284.45 *

Weight:

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The Cas9-Dead-NLS-EGFP protein is catalytically inactive ("dead") due to the D10A and H840A... more
Product information "Cas9-Dead-NLS-EGFP"

The Cas9-Dead-NLS-EGFP protein is catalytically inactive ("dead") due to the D10A and H840A substitutions. The NLS is followed by a C-terminal enhanced green-fluorescence protein (EGFP) sequence. The EGFP-tag offers many application possibilities, e.g. tracking of Cas9-Dead-NLS-EGFP.

Excitation/emission maxima: 488 nm/507 nm

Genaxxon offers a wide range of different recombinant Streptococcus (S.) pyogenes Cas9 proteins, all of which are highly pure and effective. Fusion with a nuclear localization signal (NLS) ensures targeting of the Cas9 proteins to the nucleus. Successful genome editing by Cas9-NLS proteins has been confirmed in a wide variety of mouse and human cell lines, and organisms, including zebrafish, Xenopus and marine plankton.

Other Cas9-NLS proteins from Genaxxon: Cas9-NLS (nuclease, wildtype) >, Cas9-NLS-tagRFP (nuclease, with red fluorescent protein tag) >; Cas9-Nickase-NLS (nickase) >; Cas9-Dead-NLS (none) >

Transfection reagent for the co-transfection RNA/protein, specially designed for use in CRISPR/Cas9 systems: CRISPRfect E >.

More products around "Cas9-Dead-NLS-EGFP"
Our comment on "Cas9-Dead-NLS-EGFP"
Specific genome targeting without DNA cleavage. Labelling of target sites.
Solution of Cas9-Dead-NLS protein. Concentration: 0.5µg/µL (Higher concentrations are available... more
 

Technical Data:

Solution of Cas9-Dead-NLS protein. Concentration: 0.5µg/µL (Higher concentrations are available on request). MW of protein: 188 kDa. Storage Buffer: 20 mM HEPES pH 7.25, 150 mM KCl, 1 mM DTT.

C-terminus NLS from Simian virus 40 (PKKKRKV) near C-terminus followed by EGFP-tag (enhanced green fluorescent protein tag).

D10A and H840A substitutions (catalytically inactive Cas9 protein).

application:

CRISPR

Source

E.coli

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 34-16-04-90
Dokumente - Protokolle - Downloads more

Dokumente - Protokolle - Downloads

Here you will find information and further literature on Cas9-Dead-NLS-EGFP. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.

 
 
 
Literatur .... more

Referenzen..

Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.

Quelle/Source: NCBI PubMed >

 

In vivo topology converts competition for cell-matrix adhesion into directional migration
Fernanda Bajanca, Nadège Gouignard, Charlotte Colle, Maddy Parsons, Roberto Mayor, Eric Theveneau
Nat Commun. 2019; 10: 1518. Published online 2019 Apr 3. doi: 10.1038/s41467-019-09548-5
PMCID: PMC6447549

 
 
 
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