Geneticin (G418) - 50mg/mL



Order number: M3118.0050

Shipping: shipped at RT, store at +2°C to +8°C
CAS-Nr: 108321-42-2

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Geneticin, often also called G418, is an aminoglycoside antibiotic that inhibits protein... more
Product information "Geneticin (G418) - 50mg/mL"

Geneticin, often also called G418, is an aminoglycoside antibiotic that inhibits protein synthesis in mammalian cells by interfering with ribosomal function. Geneticin (G418) shows a structure similar to gentamycin and kanamycin and finds application in the selection of stably transformed cells expressing the neomycin resistance gene (aminoglycoside) of transposons Tn 5 or Tn 601.

Because G418 (Geneticin) is toxic for many cells, the optimum concentration for each cell type has to be determined. It is usually between 50ug/mL and 1000μg/mL. At a concentration of 500μg/mL, for example, 70% of HepG2 cells are killed after incubation for one week and 100% will stop their growth. Since potency of the antibiotic can vary from batch to batch, it is recommended to create large stocks of a batch tested to standardize the system.

Cells divide still once or twice in the presence of the lethal dose of G418 (Geneticin), so that a selection effect will be visible only after several days. Geneticin is a registered trademark of Gibco, a Division of Life Technologies.

Genaxxon bioscience offers a broad range of Antibiotics and Antimycotics as powder or solutions.

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Our comment on "Geneticin (G418) - 50mg/mL"
G418 inhibits protein synthesis in mammalian cells by interfering with ribosomal function.
Specifications: 50 mg/mL sterile filtered solution C20H40N4O10x2H2SO4 MW = 692.7 g/mol Liquid... more
 

Technical Data:

Specifications:
50 mg/mL sterile filtered solution
C20H40N4O10x2H2SO4
MW = 692.7 g/mol
Liquid formulation of Cat#: M3117

Harmful: LD50 of <2000 mg/kg. May be carcinogen/teratogenic.

application:

selection of stably transformed cells expressing the neomycin resistance gene (aminoglycoside) of transposons Tn 5 or Tn 601.

Sicherheits Hinweise / Safety

H-Sätze: H301
R-Sätze: R61
Gefahrstoffkürzel: T

Klassifizierungen / Classification

CAS-Nr: 108321-42-2
eclass-Nr: 32-17-01-90
Dokumente - Protokolle - Downloads more

Dokumente - Protokolle - Downloads

Here you will find information and further literature on Geneticin (G418) - 50mg/mL. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.

 
 
 
Literatur .... more

Referenzen..

Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.

Quelle/Source: NCBI PubMed >

 

IL-23R Signaling Plays No Role in Myocardial Infarction
Erika Engelowski, Nastaran Fazel Modares, Simone Gorressen, Pascal Bouvain, Dominik Semmler, Christina Alter, Zhaoping Ding, Ulrich Flögel, Jürgen Schrader, Haifeng Xu, Philipp A. Lang, Jens Fischer, Doreen M. Floss, Jürgen Scheller
Sci Rep. 2018; 8: 17078. Published online 2018 Nov 20. doi: 10.1038/s41598-018-35188-8
PMCID: PMC6244091

Defining the functional binding sites of interleukin 12 receptor β1 and interleukin 23 receptor to Janus kinases

Doreen M. Floss, Tobias Klöcker, Jutta Schröder, Larissa Lamertz, Simone Mrotzek, Birgit Strobl, Heike Hermanns, Jürgen Scheller

Mol Biol Cell. 2016 Jul 15; 27(14): 2301–2316. doi: 10.1091/mbc.E14-12-1645

PMCID: PMC4945146

MicroRNA-34a promotes genomic instability by a broad suppression of genome maintenance mechanisms downstream of the oncogene KSHV-vGPCR

Claudia J. Krause, Oliver Popp, Nanthakumar Thirunarayanan, Gunnar Dittmar, Martin Lipp, Gerd Müller

Oncotarget. 2016 Mar 1; 7(9): 10414–10432. Published online 2016 Feb 8. doi: 10.18632/oncotarget.7248

PMCID: PMC4891129

Non-Canonical Interleukin 23 Receptor Complex Assembly: P40 PROTEIN RECRUITS INTERLEUKIN 12 RECEPTOR β1 VIA SITE II AND INDUCES P19/INTERLEUKIN 23 RECEPTOR INTERACTION VIA SITE III

Jutta Schröder, Jens M. Moll, Paul Baran, Joachim Grötzinger, Jürgen Scheller, Doreen M. Floss

J Biol Chem. 2015 Jan 2; 290(1): 359–370. Published online 2014 Nov 4. doi: 10.1074/jbc.M114.617597

PMCID: PMC4281739

Alternative Intronic Polyadenylation Generates the Interleukin-6 Trans-signaling Inhibitor sgp130-E10

Jan Sommer, Christoph Garbers, Janina Wolf, Ahmad Trad, Jens M. Moll, Markus Sack, Rainer Fischer, Joachim Grötzinger, Georg H. Waetzig, Doreen M. Floss, Jürgen Scheller

J Biol Chem. 2014 Aug 8; 289(32): 22140–22150. Published online 2014 Jun 27. doi: 10.1074/jbc.M114.560938

PMCID: PMC4139227

 
 
 
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