Penicillin-Streptomycin (10000 U/mL)
Order number: M3140.0100
Shipping: Shipment: on wet ice. Store at -20°C. For laboratory usage only! Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.
*Prices plus VAT plus shipping costs
Pen-Strep: Penicillin G from Penicillium notatum and Streptomycin from Streptomyces griseus prevent together against gram-positive and gram-negative bacteria the bacterial contamination of cell cultures. Penicillin G acts by interfering directly with turnover of bacterial cell wall and indirectly by triggering release of enzymes that alter the cell wall. Streptomycin works by attaching to the 30S subunit of the bacterial ribosome, which leads to an inhibition of protein synthesis and the subsequent death of bacteria.
Penicillin G potassium salt is soluble in water. At +4°C or room temperature, the dry substance is stable for approximately 5 years. The buffered solution (pH4,5-7) will loose 5% activity after 20 days at +4°C respective 20% after 60 days at +4°C. At room temperature, reduction in activity will be 5% after only 48 hours, 10% after 7 days, and 20% to 50% after 14 days. In an unbuffered solution stability is considerably lower (about 3-7 days at +4°C).
Pen-Strep: Penicillin G and Streptomycin against bacterial contamination of cell cultures.
Genaxxon bioscience offers besides Pen-Strep a broad range of Antibiotics and Antimycotics as powder or solutions, e.g., Geneticin G418 or Gentamycin sulfate.
• 10,000 mg/mL Streptomycin • 10 mg/mL Penicillin • 0.9% NaCl • effective against gram + and gram - bacteria • can be used prophylactic • Streptomycin-Penicillin is a broad band antibiotic • inhibits protein biosynthesis (streptomycin) • inhibits cell wall synthesis (penicillin)
Technical Data:
Specifications:
Penicillin G content: 9,000 – 11,000 U/mL
Streptomycin content: 9 – 11 mg/mL
0.9% NaCl (physiological NaCl solution)
pH: 6.0 – 7.0
Osmolality: 280 – 400 mOsmol/kg
Endotoxin: <0.1 EU/mL
Bioburden: 0 CFU/mL
Shelf-life at 37°C: 5 days
sterile filtered solution for cell culture applications
application:
cell culture applicationsSource
Steptomycin is isolated from Actinomycetes Streptomyces griseusSicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-19-90-90Dokumente - Protokolle - Downloads
Here you will find information and further literature on Penicillin-Streptomycin (10000 U/mL). For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.
Dokumente - Protokolle - Downloads
Äquivalent/Alternative zu: Biochrom Cat.-no. A 2213, oder zu Bio&Sell Nr. BS.A2213.
Change in colour of the Pen/Strep solution:
Normally this change in colour does not mean that you can not use the solution anymore. There are different reasons for it.
1. Chemical reaction of Penicillin and oxygen which will form a substance that absorbs light to get a pale yellow colour. But this does not mean that the majority of the Penicillin is degraded. THere is still enough antibiotic in the solution.
2. The other point is, sometimes, that the Streptomycin source does still contain some pigments from the strain from which Streptomycin is isolated. These pigments may also undergo a reaction with oxygen that turns them more yellowish.
In any case you feel not good about the yellowish colour you should discard it, at this will be the only possibility to have no uncertainty for explanations of further results.
Referenzen..
Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieses Genaxxonprodukts vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
bFGF-mediated pluripotency maintenance in human induced pluripotent stem cells is associated with NRAS-MAPK signaling
Fereshteh Haghighi, Julia Dahlmann, Saeideh Nakhaei-Rad, Alexander Lang, Ingo Kutschka, Martin Zenker, George Kensah, Roland P. Piekorz, Mohammad Reza Ahmadian
Cell Commun Signal. 2018; 16: 96. Published online 2018 Dec 5. doi: 10.1186/s12964-018-0307-1
PMCID: PMC6282345
IL-23R Signaling Plays No Role in Myocardial Infarction
Erika Engelowski, Nastaran Fazel Modares, Simone Gorressen, Pascal Bouvain, Dominik Semmler, Christina Alter, Zhaoping Ding, Ulrich Flögel, Jürgen Schrader, Haifeng Xu, Philipp A. Lang, Jens Fischer, Doreen M. Floss, Jürgen Scheller
Sci Rep. 2018; 8: 17078. Published online 2018 Nov 20. doi: 10.1038/s41598-018-35188-8
PMCID: PMC6244091
Utku Kürşat Ercan, Fatma İbiş, Caner Dikyol, Nesrin Horzum, Ozan Karaman, Çağla Yıldırım, Elif Çukur, Emine Afra Demirci
PLoS One. 2018; 13(9): e0202703. Published online 2018 Sep 5. doi: 10.1371/journal.pone.0202703
PMCID: PMC6124751
Synthetic cytokine receptors transmit biological signals using artificial ligands
Erika Engelowski, Artur Schneider, Manuel Franke, Haifeng Xu, Ramona Clemen, Alexander Lang, Paul Baran, Christian Binsch, Birgit Knebel, Hadi Al-Hasani, Jens M. Moll, Doreen M. Floß, Philipp A. Lang, Jürgen Scheller
Nat Commun. 2018; 9: 2034. Published online 2018 May 23. doi: 10.1038/s41467-018-04454-8
PMCID: PMC5964073
D. M. Floss, M. Schönberg, M. Franke, F. C. Horstmeier, E. Engelowski, A. Schneider, E. M. Rosenfeldt, J. Scheller
Sci Rep. 2017; 7: 15172. Published online 2017 Nov 9. doi: 10.1038/s41598-017-15173-3
PMCID: PMC5680241
Thorben M. Hummel, Theresa Ackfeld, Marco Schönberg, Gregor Ciupka, Falk Schulz, Anne Oberdoerster, Joachim Grötzinger, Jürgen Scheller, Doreen M. Floss
Mol Cell Biol. 2017 Sep 1; 37(17): e00014-17. Prepublished online 2017 Jun 19. Published online 2017 Aug 11. doi: 10.1128/MCB.00014-17
PMCID: PMC5559674
Doreen M. Floss, Tobias Klöcker, Jutta Schröder, Larissa Lamertz, Simone Mrotzek, Birgit Strobl, Heike Hermanns, Jürgen Scheller
Mol Biol Cell. 2016 Jul 15; 27(14): 2301–2316. doi: 10.1091/mbc.E14-12-1645
PMCID: PMC4945146
A widespread sequence-specific mRNA decay pathway mediated by hnRNPs A1 and A2/B1
Rene Geissler, Alfred Simkin, Doreen Floss, Ravi Patel, Elizabeth A. Fogarty, Jürgen Scheller, Andrew Grimson
Genes Dev. 2016 May 1; 30(9): 1070–1085. doi: 10.1101/gad.277392.116
PMCID: PMC4863738
Claudia J. Krause, Oliver Popp, Nanthakumar Thirunarayanan, Gunnar Dittmar, Martin Lipp, Gerd Müller
Oncotarget. 2016 Mar 1; 7(9): 10414–10432. Published online 2016 Feb 8. doi: 10.18632/oncotarget.7248
PMCID: PMC4891129
Soren Hayrabedyan, Krassimira Todorova, Asma Jabeen, Gergana Metodieva, Stavri Toshkov, Metodi V. Metodiev, Milcho Mincheff, Nelson Fernández
Sci Rep. 2016; 6: 18896. Published online 2016 Jan 8. doi: 10.1038/srep18896
PMCID: PMC4705529
Ratna Ghosh, Marília K. F. de Campos, Jin Huang, Seong K. Huh, Adam Orlowski, Yuan Yang, Ashutosh Tripathi, Aaron Nile, Hsin-Chieh Lee, Marek Dynowski, Helen Schäfer, Tomasz Róg, Marta G. Lete, Hasna Ahyayauch, Alicia Alonso, Ilpo Vattulainen, Tatyana I. Igumenova, Gabriel Schaaf, Vytas A. Bankaitis
Mol Biol Cell. 2015 May 1; 26(9): 1764–1781. doi: 10.1091/mbc.E14-10-1475
PMCID: PMC4436786
Jutta Schröder, Jens M. Moll, Paul Baran, Joachim Grötzinger, Jürgen Scheller, Doreen M. Floss
J Biol Chem. 2015 Jan 2; 290(1): 359–370. Published online 2014 Nov 4. doi: 10.1074/jbc.M114.617597
PMCID: PMC4281739
Jan Sommer, Christoph Garbers, Janina Wolf, Ahmad Trad, Jens M. Moll, Markus Sack, Rainer Fischer, Joachim Grötzinger, Georg H. Waetzig, Doreen M. Floss, Jürgen Scheller
J Biol Chem. 2014 Aug 8; 289(32): 22140–22150. Published online 2014 Jun 27. doi: 10.1074/jbc.M114.560938
PMCID: PMC4139227
Identification of autophagy as a longevity-assurance mechanism in the aging model Podospora anserina
Laura Knuppertz, Andrea Hamann, Francesco Pampaloni, Ernst Stelzer, Heinz D Osiewacz
Autophagy. 2014 May; 10(5): 822–834. Published online 2014 Feb 27. doi: 10.4161/auto.28148
PMCID: PMC5119060
PKCβ Phosphorylates PI3Kγ to Activate It and Release It from GPCR Control
Romy Walser, John E. Burke, Elena Gogvadze, Thomas Bohnacker, Xuxiao Zhang, Daniel Hess, Peter Küenzi, Michael Leitges, Emilio Hirsch, Roger L. Williams, Muriel Laffargue, Matthias P. Wymann
PLoS Biol. 2013 Jun; 11(6): e1001587. Published online 2013 Jun 25. doi: 10.1371/journal.pbio.1001587
PMCID: PMC3692425
Doreen M. Floss, Simone Mrotzek, Tobias Klöcker, Jutta Schröder, Joachim Grötzinger, Stefan Rose-John, Jürgen Scheller
J Biol Chem. 2013 Jul 5; 288(27): 19386–19400. Published online 2013 May 14. doi: 10.1074/jbc.M112.432153
PMCID: PMC3707643
Mutational analysis of a phenazine biosynthetic gene cluster in Streptomyces anulatus 9663
Orwah Saleh, Katrin Flinspach, Lucia Westrich, Andreas Kulik, Bertolt Gust, Hans-Peter Fiedler, Lutz Heide
Beilstein J Org Chem. 2012; 8: 501–513. Published online 2012 Apr 4. doi: 10.3762/bjoc.8.57
PMCID: PMC3326630
Kerstin Seeger, Katrin Flinspach, Elisa Haug‐Schifferdecker, Andreas Kulik, Bertolt Gust, Hans‐Peter Fiedler, Lutz Heide
Microb Biotechnol. 2011 Mar; 4(2): 252–262. Published online 2011 Feb 22. doi: 10.1111/j.1751-7915.2010.00234.x
PMCID: PMC3818865
Orwah Saleh, Bertolt Gust, Björn Boll, Hans-Peter Fiedler, Lutz Heide
J Biol Chem. 2009 May 22; 284(21): 14439–14447. doi: 10.1074/jbc.M901312200
PMCID: PMC2682892
