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Accutase® as a cell detachment solution of proteolytic and collagenolytic enzymes useful for the routine detachment of cells from standard tissue culture plastic ware and adhesion coated plastic ware. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue. It is much better suited for cell detachment than trypsin. Proven effective in detaching primary fibroblasts, endothelial cells, neurons, tumor cell lines, and insect cells.
Accutase® does not contain mammalian or bacterial derived products. For that reason Accutase® virus or endotoxin contaminations are excluded. Once thawed Accutase® can be used for about 1 week if stored at +2°C to +8°C. Shelf-life at -20°C is 12 months.
Note: Accutase is a registered trademark of Innovative Cell Technologies, Inc.
Ready-to-use liquid consisting of a proprietary mixture of proteolytic and collagenolytic enzymes
Prepared in Dulbecco′s PBS (0.2g/L KCl, 0.2g/L KH2PO4, 8g/L NaCl, and 1.15g/L Na2HPO4) containing 0.5mM EDTA and Phenol Red.
pH: 6.6 - 7.6
Sterile filtered solution
Suitable for cell culture
application:for use in dissociation of cells from standard and adhesion-coated plasticware
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 34-16-05-12
Dokumente - Protokolle - Downloads
Here you will find information and further literature on Accutase® Cell Detachment Solution. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: email@example.com or phone: +49 731 3608 123.