Trypsin powder from porcine pancreas (1:250)

Trypsin from porcine pancreas


Order number: C4264.0025

Shipping: Shipment: not cooled. Store at +15°C to +30°C. For laboratory usage only!
CAS-Nr: 9002-07-7

Delivery time: 3- 8 working days
For detailed information on the delivery date, please contact Genaxxon.

€128.87 *

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Trypsin 1:250 from porcine pancreas (240 - 260 USP U/mg). Contains Chymotrypsin, Elastase and... more
Product information "Trypsin powder from porcine pancreas (1:250)"

Trypsin 1:250 from porcine pancreas (240 - 260 USP U/mg). Contains Chymotrypsin, Elastase and other non proteolytic activities.

The native form of Trypsin consists of a single chain polypeptide of 223 amino acid residues, produced by the removal of the N-terminal hexapeptide from trypsinogen which is cleaved at the Lys - lle peptide bond. The sequence of amino acids is cross-linked by 6 disulfide bridges. Trypsin is a member of the serine protease family.

Application
For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestionsns. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.

Serine protease inhibitors, including DFP, TLCK, APMSF, AEBSEF, and aprotinin, amongst others, will inhibit Trypsin.

Preparation of Trypsin solutions
This product is a lyophilized powder which can be easily dissolved in aqueous solutions, e.g. in Hank′s Balanced Salt Solution at 25mg/mL. For applications that require EDTA, solubilizing trypsin should be done with a buffered salt solution contaiing no Ca2+ or Mg2+.

Caution: Solutions in 1 mM HCl are stable for 1 year in aliquots and stored at -20°C. The presence of Ca2+ will also diminish the self-autolysis of trypsin and maintain its stability in solution. Trypsin will also retain most of its activity in 2.0 M urea, 2.0 M guanidine HCl, or 0.1% (w/v) SDS.

Unit Definition:
One BAEE unit will produce a ΔA253 of 0.001 per min at pH 7.6 at 25° C using BAEE as substrate. One BTEE unit = 320 ATEE units. Reaction volume = 3.2 mL (1 cm light path).

More products around "Trypsin powder from porcine pancreas (1:250)"
Specifications: Trypsin content (BAEE): >225 USP U/mg Chymotrypsin content (ATEE): 75 - 180... more
 

Technical Data:

Specifications:
Trypsin content (BAEE): >225 USP U/mg
Chymotrypsin content (ATEE): 75 - 180 USP U/mg
Appearance: white-off powder
Germs: max. 10000/g
Moulds and Yeast: max. 100 counts/g
Salmonella species: absence/10g
Staphylococcus aureus: absence/g
Escherichia Coli: absence/g
Loss on drying: max. 5% (60°C)
MW = 23.8 kDa

application:

For trypsin digestion of peptides, use a ratio of about 1:100 to 1:20 for trypsin:peptide. The typical use for this product is in removing adherent cells from a culture surface. The concentration of trypsin necessary to dislodge cells from their substrate is dependent primarily on the cell type and the age of the culture. Trypsins have also been used for the re-suspension of cells during cell culture, in proteomics research for digestion of proteins and in various in-gel digestionsns. Additional applications include assessing crystallization by membrane-based techniques and in a study to determine that protein folding rates and yields can be limited by the presence of kinetic traps.

Unit Definition:

One BAEE unit will produce a ΔA253 of 0.001 per min at pH 7.6 at 25° C using BAEE (N-a-benzoyl-L-arginine ethyl ester) as substrate. One BTEE unit = 320 ATEE units. Reaction volume = 3.2 mL (1 cm light path).

Source

porcine pancreas

Sicherheits Hinweise / Safety

H-Sätze: H315, H319, H334, H335
GHS-Symbole: GHS07, GHS08
R-Sätze: R36/37/38-42
Gefahrstoffkürzel: Xn

Klassifizierungen / Classification

EC-Nr: 232-650-8
CAS-Nr: 9002-07-7
eclass-Nr: 32-16-08-90
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Here you will find information and further literature on Trypsin powder from porcine pancreas (1:250). For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.