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Collagenase Type II

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Manufacturer: Genaxxon bioscience



Order number: C4341.0100

shipped at RT, store at +2°C to +8°C

CAS-Nr: 9001-12-1
 

Ready to ship today,
Delivery time appr. 1-3 workdays

€99.52 *
 
 
 
 
 

Product information "Collagenase Type II"

Genaxxon Collagenase from Clostridium histolyticum is an enzymatic mixture from Collagenase, Clostripain (cleaves peptides preferentially at the carboxyl side of arginine residues), several tryptic (acts preferentially at the carboxyl side of arginine and lysine residues) and chymotryptic activities (non-specific). Collagenase Typ II shows a high Clostripain acitivity while the tryptic activity is comparable to the one from Collagenase Typ I. Typ II Collagenase is recommended for preparation of cells from liver, bone, thyroid gland, heart and salivary gland. The specific activity is 125 to 250 Mandl-Units per mg dry substance.

General description:
The treatment of tissue with collagenase causes the careful, selective degradation of the intercellular matrix, and does not affect the growth of the cells. Collagenase offered by Genaxxon bioscience is a mixture of various proteolytic active enzymes and needs Calcium ions for its catalytic activity and binding to the collagen molecule.

For optimal results, a well balanced mixture of proteolytic enzymes is necessary. Four different collagenases, Type I, Type II, Type III and Type IV, are available for this. Type IV is usually used together with other enzymes, such as trypsin, elastase or hyaluronidase. Trypsin or Trypsin/EDTA conventionally used in the cell culture dgrades the matrix only slowly and can cause irreversible damage to the released cells. Therefore, we recommend the use of Accutase instead of trypsin or trypsin/EDTA.

The enzyme is reversibly inactivated at high pH values and irreversibly inactivated at low pH values.

Inhibitors of collagenase include cysteine, EDTA, o-phenanthroline, 8-hydroxyquinoline-5-sulfonate, bipyridyl and 2,3-dimercaptopropanol. Collagenase is NOT inhibited by diisopropylphosphorofluoridate.

Lyphilised Collagenase should be stored at +2°C to +8°C and remain stable without loss of activity for at least three years. Enzyme should be protected from moisture. Dissolved Collagenase can be aliquoted and stored at -20°C for one year.

Conversion rates of collagenase activity units: 1 PZ U/mg ~ 3.9 FALGPA U/mg - 1 PZ U/mg ~ 1000 Mandl or CDU U/mg - 1 PZ U/mg ~ 10 HP U/mg (PZ-units according to Wünsch).

 

application:

Recommended for cell preparation from epithelial and lung tissue, tissue of the suprarenal gland and adipose tissue.
 
 

Our comment on "Collagenase Type II"

High Clostripain activity while showing a tryptic activity that is comparable with that of Collagenase Type I.
 

Technical Data:

Specific activity: >125 units/mg dry weight. High Clostripain activity while showing a tryptic activity that is comparable with that of Collagenase Type I.

application:

Recommended for cell preparation from epithelial and lung tissue, tissue of the suprarenal gland and adipose tissue.

Source

Clostridium histolyticum

Sicherheits Hinweise / Safety

H-Sätze: H317, H334
GHS-Symbole: GHS08

Klassifizierungen / Classification

EC-Nr: 232-582-9
CAS-Nr: 9001-12-1
eclass-Nr: 32-16-04-10

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