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Zymolyase®, produced by a submerged culture of Arthrobacter luteus, has strong lytic activity against living yeast cell walls to produce protoplast or spheroplast of various strains of yeast cells. An essential enzyme for the lytic activity of Zymolyase® is β-1,3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers with β-1,3-linkages and releases specifically laminaripentaose as the main and minimum product unit. Reported to be useful for lysis of Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, and Schwanniomyces species.
There are two preparations of Zymolyase®, Zymolyase®-20T (C4352) and Zymolyase®-100T (C4366) , having lytic activity of 20,000 units/g and 100,000 units/g respectively. The activity was tested towards brewer’s yeast cells (Saccharomyces cerevisiae, resting stage) or towar yeast cells of Saccharomyces uvarum (IFO 0565) cultured statically in malt extract medium.
Zymolyase®-20T is ammonium sulfate precipitate while Zymolyase®-100T is a further purified preparation by affinity chromatography. Lytic activity varies depending on yeast strain, growth stage of yeast, or cultural conditions. Further informations related to Zymolyase® can be obtained from the data sheet and the references given there.
Specificity (lytic spectrum): Ashbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloeckera, kluyveromyces, Lipomyces, Metschnikowia, Pichia, Pullularia, Torulopsis, Saccharomyces, Saccharomycopsis, Saccharomycodes, Schwannio.
Specific activity: 100,000 units/g (Zymolyase-T100). pH7.5 (35°C). For hydrolysis of yeast glucan (Zymolyase-T100).
application:pH7.5 (35°C): for lysis of viable yest cells pH6.5 (45°C): for hydrolysis of yeast glucan
Unit Definition:One unit of lytic activity is defined as that amount which indicates 30% of decrease in absorbance at 800nm (A800) of the reaction mixture under the following condition.
Sicherheits Hinweise / Safety
Klassifizierungen / ClassificationCAS-Nr: 37340-57-1