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Genaxxon Collagenase from Clostridium histolyticum is an enzymatic mixture from Collagenase, Clostripain (cleaves peptides preferentially at the carboxyl side of arginine residues), several tryptic (acts preferentially at the carboxyl side of arginine and lysine residues) and chymotryptic activities (non-specific). Collagenase Typ III shows normal Collagenase, but very low proteolytic activity. Typ III Collagenase is recommended for preparation of cells from mamary gland. The specific activity is 100 to 250 Mandl-Units per mg dry substance.
The treatment of tissue with collagenase causes the careful, selective degradation of the intercellular matrix, and does not affect the growth of the cells. Collagenase offered by Genaxxon bioscience is a mixture of various proteolytic active enzymes and needs Calcium ions for its catalytic activity and binding to the collagen molecule.
For optimal results, a well balanced mixture of proteolytic enzymes is necessary. Four different collagenases, Type I, Type II, Type III and Type IV, are available for this. Type IV is usually used together with other enzymes, such as Trypsin, elastase or Hyaluronidase. Trypsin or Trypsin/EDTA conventionally used in the cell culture dgrades the matrix only slowly and can cause irreversible damage to the released cells. Therefore, we recommend Accutase instead of trypsin or trypsin/EDTA.
The enzyme is reversibly inactivated at high pH values and irreversibly inactivated at low pH values.
Inhibitors of collagenase include cysteine, EDTA, o-phenanthroline, 8-hydroxyquinoline-5-sulfonate, bipyridyl and 2,3-dimercaptopropanol. Collagenase is NOT inhibited by diisopropylphosphorofluoridate.
Lyphilised Collagenase should be stored at +2°C to +8°C and remain stable without loss of activity for at least three years. Enzyme should be protected from moisture. Dissolved Collagenase can be aliquoted and stored at -20°C for one year.
Conversion rates of collagenase activity units: 1 PZ U/mg ~ 3.9 FALGPA U/mg - 1 PZ U/mg ~ 1000 Mandl or CDU U/mg - 1 PZ U/mg ~ 10 HP U/mg (PZ-units according to Wünsch).
Very low proteolytic activity, normal collagenase activity.
Specific acitivity: >125 units/mg dry weight. Very low proteolytic activity, normal collagenase activity. (EC 184.108.40.206)
application:Recommended for cell preparation from epithelial and lung tissue, tissue of the suprarenal gland and adipose tissue.
Sicherheits Hinweise / SafetyH-Sätze: H317, H334
Klassifizierungen / ClassificationEC-Nr: 232-582-9