Lympho-Paque - Cell separation medium

Lympho-Paque is a sterile, ready to use medium for isolation of lymphocytes in high yield from peripheral blood using a simple high speed centrifugation procedure. Density: 1.077 g/mL. Lympho-Paque maintains the viability and a representative distribution of B and T lymphocytes.

Often the isolation of cells or sub-cellular partiles is the first step in gene expression research or in diagnostic examinations. Apart from bio-specific separation methods physical separation methods are most commonly used which utilize separation by physical differences such as size and charge of the particles to be separated. For this purpose so called density gradient solutions are used. These solutions have to meet the following criteria: 1. They must be able to form a density gradient over the desired density range. 2. The desired pH and osmolality must be easily adjustable. 3. The solution mus not be too viscous in case of high density. 4. They must not cause any functional or morphological changes in biological materials. 5. They must not penetrate biological membranes. The Genaxxon Lymphocyte separation medium enables high yield recovery of lymphocytes and other mononuclear cells from anticoagulated whole blood through a simple centrifugation step. The erythrocytes aggregate and the granulocytes become slightly hypertonic, increasing their sedimentation rate, resulting in a pellet at the bottom of the centrifuge tube. Lymphocytes and other mononuclear cells remain in the interphase.