Alpha MEM Eagle with nucleosides, with L-Glutamine, with Glucose

MEM α (Minimum Essential Medium α) is widely used for mammalian cell culture as well as selection for transfected DHFR-negative cells. MEM α can be used with a variety of suspension and adherent mammalian cells, including keratinocytes, primary rat astrocytes, and human melanoma cells. We offer a variety of MEM α modifications for a range of cell culture applications.

MEM α is a modification of Minimum Essential Medium (MEM) that contains non-essential amino acids, sodium pyruvate, lipoic acid, vitamin B12, biotin, and ascorbic acid. MEM α is available without nucleosides for use as a selection medium for DG44 and other DHFR-negative cells. This product is made with Earle’s salts. MEM α contains no proteins, lipids, or growth factors. Therefore, MEM α requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). MEM α uses a sodium bicarbonate buffer system (2.2g/L), and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Alpha MEM Eagle (with Earles salts) with Glutamine, with Glucose, with Ribonucleosids and Deoxyribonucleosides, with 2.2g/L NaHCO3, sterile filtered. Special preparation. Order size: min. 20 x 500mL. If you wish, you can choose all incredients as you need without changing the price per bottle. Just let us know your needs.