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Dulbecco's Modified Eagle Medium (DMEM), 4.5g/L glucose, no glutamine, no lysine, no arginine, no methionine is a basal cell culture medium for use during SILAC™ protein labeling with stable isotopic labeled lysine and/or arginine. Special preparation. Minimum order size: 20 x 500mL.
DMEM, intrinsically developed for the cultivation of murine embryonic cells, is tailor-made for the cultivation of a broad range of cells, especially if the media is supplemented with FCS. DMEM is an Eagle Medium modification with the four-fold content of amino acids and vitamin. While DMEM with 1.0g/L Glucose is the standard media, DMEM with 4.5g/L Glucose is for cells which have a high energy demand. is a widely used basal medium for supporting the growth of many different mammalian cells.
Cells successfully cultured in DMEM include primary fibroblasts, neurons, glial cells, HUVECs and smooth muscle cells, as well as cell lines such as HeLa, 293, Cos-7, and PC-12.
DMEM contains 4 times the concentration of amino acids and vitamins than the original Eagle's Minimal Essential Medium. It was originally formulated with low glucose (1.0g/L) and Sodium pyruvate, but is often used with higher glucose levels (4.5g/L).
DMEM contains no proteins, lipids, or growth factors. Therefore, DMEM requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). As DMEM uses a sodium bicarbonate buffer system (3.7g/L), a 5–10% CO2 environment is necessary to maintain physiological pH.
Genaxxon offers a variety of DMEM modifications for a range of cell culture applications. If you can't find the medium you are looking for we will be glad to prepare the requested medium you do need. Just let us know your needs: email@example.com.
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with 4.5g/L Glucose
without Sodium pyruvate
with Phenol red
with 3.7g/L NaHCO3
sterile filtered solution for use in cell culture
application:for use in cell culture applications
Sicherheits Hinweise / Safety
Klassifizierungen / Classificationeclass-Nr: 32-16-08-01
Dokumente - Protokolle - Downloads
Here you will find information and further literature on DMEM w/o Glutamine, w/o Arginine, w/o Lysin, w/o Methionine with 4.5g/L Glucose. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: firstname.lastname@example.org or phone: +49 731 3608 123.