DMEM:F12, 1:1 Mix with stable glutamine, with HEPES - 500 mL

DMEM:F12, 1:1 mixture without L-glutamine, with 15mM Hepes buffer and 1.2/L NaHCO3. Can be supplemented by adding 7% NaHCO3 (> C4213) and/or stable glutamine (> C4282) and thus adapted to the corresponding special applications.

DMEM/F-12 is a 1:1 mixture of Dulbecco's Modified Eagle Medium and Ham's F-12. It combines the high levels of glucose, amino acids and vitamins from DMEM with the various components of Ham's F-12. It is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.

DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Genaxxon offers a variety of DMEM modifications for a range of cell culture applications. If you can't find the medium you are looking for we will be glad to prepare the requested medium you do need. Just let us know your needs: info@genaxxon.com.

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