Basal Medium (Eagle) with EBSS, without L-glutamine

Basal Medium (Eagle) with EBSS without Glutamine, without Glucose, with Phenol red, with 2.2g/L NaHCO3. 

In the fifties of the last century it became clear that mammalian cells need not only the 10 essential amino acids, but also Cystine, Tyrosine and Glutamine. In addition to these three amino acids, BME includes also eight B-vitamins. Originally BME was used for the cultivation of murine L-cells and HeLa-cells. With its many variations it is used in many fields of cell culture and applications today. Along with the cultivation of normal mammalian cells BME is very suitable for transformed cells.

BME contains no proteins, lipids, or growth factors. Therefore, BME requires supplementation, commonly with 10% Fetal Bovine Serum (FBS). As this BME uses a sodium bicarbonate buffer system (2.2g/L), a 5–10% CO2 environment is necessary to maintain physiological pH.

Genaxxon offers a variety of cell culture media modifications for a range of cell culture applications. If you can't find the medium you are looking for we will be glad to prepare the requested medium you do need. Just let us know your needs: info@genaxxon.com.

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