DMEM:F12, 1:1 Mix with L-Glutamine, with 15mM HEPES

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Volume

500 mL

Product number: C4023.0500
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Delivery time 1-2 workdays

Shipment: not cooled. Store at +2°C to +8°C. For laboratory usage only!

Product information "DMEM:F12, 1:1 Mix with L-Glutamine, with 15mM HEPES"

DMEM:F12, 1:1 Mix with L-Glutamine, with 15mM HEPES, with Phenol red, with 1.2g/L NaHCO3.

DMEM/F-12 is a 1:1 mixture of Dulbecco's Modified Eagle Medium and Ham's F-12. It combines the high levels of glucose, amino acids and vitamins from DMEM with the various components of Ham's F-12. It is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.

DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Genaxxon offers a variety of DMEM modifications for a range of cell culture applications. If you can't find the medium you are looking for we will be glad to prepare the requested medium you do need. Just let us know your needs: info@genaxxon.com.

Genaxxon offers also other products as our broad range of Antibiotcs > - Amphotericin B > - Antibiotic Antimycotic Solution > - Doxycycline > - Geneticin disulfate (G418) > - Gentamycin sulfate > - Hygromycin B > - Kanamycin > - Penicillin/Streptomycin >.

Specification:
with L-Glutamine
with Glucose
with Phenol red
with Sodium pyruvate
with 15mM HEPES
with 1.2g/L NaHCO3

sterile filtered solution for cell culture applications

Applikation / Application:

for use in cell culture applications

Einheiten / Units:

Quelle / Source:

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

eclass-Nr: 32-16-08-01

Documents - Protocols - Downloads :

Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.

Documents:

Protocols
Product description
General Data 1

Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Source: NCBI PubMed

Hier finden Sie Artikel und Literaturzitate, in denen die Autoren auf die hohe Qualität dieser Genaxxonprodukte vertrauen.
Listed below are articles and references, in which the authors trust in the high quality of these Genaxxon products.

Quelle/Source: NCBI PubMed >

Ciliostasis of airway epithelial cells facilitates influenza A virus infection

Yuguang Fu, Jie Tong, Fandan Meng, Doris Hoeltig, Guangliang Liu, Xiangping Yin, Georg Herrler

Vet Res. 2018; 49: 65. Published online 2018 Jul 18. doi: 10.1186/s13567-018-0568-0

PMCID: PMC6052543

Therapeutic Effect of a Novel Phosphatidylinositol-3-Kinase δ Inhibitor in Experimental Epidermolysis Bullosa Acquisita

Hiroshi Koga, Anika Kasprick, Rosa López, Mariona Aulí, Mercè Pont, Núria Godessart, Detlef Zillikens, Katja Bieber, Ralf J. Ludwig, Cristina Balagué

Front Immunol. 2018; 9: 1558. Published online 2018 Jul 12. doi: 10.3389/fimmu.2018.01558

PMCID: PMC6052048

Combined treatment with Metformin and 2-deoxy glucose induces detachment of viable MDA-MB-231 breast cancer cells in vitro

Maruša Bizjak, Petra Malavašič, Klemen Dolinar, Jelka Pohar, Sergej Pirkmajer, Mojca Pavlin

Sci Rep. 2017; 7: 1761. Published online 2017 May 11. doi: 10.1038/s41598-017-01801-5

PMCID: PMC5431940

A liver stress-endocrine nexus promotes metabolic integrity during dietary protein dilution

Adriano Maida, Annika Zota, Kim A. Sjøberg, Jonas Schumacher, Tjeerd P. Sijmonsma, Anja Pfenninger, Marie M. Christensen, Thomas Gantert, Jessica Fuhrmeister, Ulrike Rothermel, Dieter Schmoll, Mathias Heikenwälder, Juan L. Iovanna, Kerstin Stemmer, Bente Kiens, Stephan Herzig, Adam J. Rose

J Clin Invest. 2016 Sep 1; 126(9): 3263–3278. Published online 2016 Aug 22. doi: 10.1172/JCI85946

PMCID: PMC5004939

Effects of free amino acids on cytokine secretion and proliferative activity of feline T cells in an in vitro study using the cell line MYA-1

Nadine Paßlack, Marcus G. Doherr, Jürgen Zentek

Cytotechnology. 2016 Oct; 68(5): 1949–1961. Published online 2016 Aug 10. doi: 10.1007/s10616-016-0008-9

PMCID: PMC5023574

GCN2 contributes to mTORC1 inhibition by leucine deprivation through an ATF4 independent mechanism

Julien Averous, Sarah Lambert-Langlais, Florent Mesclon, Valérie Carraro, Laurent Parry, Céline Jousse, Alain Bruhat, Anne-Catherine Maurin, Philippe Pierre, Christopher G. Proud, Pierre Fafournoux

Sci Rep. 2016; 6: 27698. Published online 2016 Jun 14. doi: 10.1038/srep27698

PMCID: PMC4906353

Medium Renewal Blocks Anti-Proliferative Effects of Metformin in Cultured MDA-MB-231 Breast Cancer Cells

Maruša Rajh, Klemen Dolinar, Katarina Miš, Mojca Pavlin, Sergej Pirkmajer

PLoS One. 2016; 11(5): e0154747. Published online 2016 May 2. doi: 10.1371/journal.pone.0154747

PMCID: PMC4852933

Annexin A1 sustains tumor metabolism and cellular proliferation upon stable loss of HIF1A

Nadine Rohwer, Fabian Bindel, Christina Grimm, Suling J. Lin, Jessica Wappler, Bertram Klinger, Nils Blüthgen, Ilona Du Bois, Bernd Schmeck, Hans Lehrach, Marjo de Graauw, Emanuel Goncalves, Julio Saez-Rodriguez, Patrick Tan, Heike I. Grabsch, Alessandro Prigione, Stefan Kempa, Thorsten Cramer

Oncotarget. 2016 Feb 9; 7(6): 6693–6710. Published online 2015 Dec 29. doi: 10.18632/oncotarget.6793

PMCID: PMC4872743

Neurotoxic 1-deoxysphingolipids and paclitaxel-induced peripheral neuropathy

Rita Kramer, Jacek Bielawski, Emily Kistner-Griffin, Alaa Othman, Irina Alecu, Daniela Ernst, Drew Kornhauser, Thorsten Hornemann, Stefka Spassieva

FASEB J. 2015 Nov; 29(11): 4461–4472. Published online 2015 Jul 21. doi: 10.1096/fj.15-272567

PMCID: PMC4608911

Sociality in Escherichia coli: Enterochelin Is a Private Good at Low Cell Density and Can Be Shared at High Cell Density

Rebecca L. Scholz, E. Peter Greenberg

J Bacteriol. 2015 Jul; 197(13): 2122–2128. Prepublished online 2015 Mar 2. Published online 2015 Jun 3. doi: 10.1128/JB.02596-14

PMCID: PMC4455259

Novel HSAN1 Mutation in Serine Palmitoyltransferase Resides at a Putative Phosphorylation Site That Is Involved in Regulating Substrate Specificity

Daniela Ernst, Sinéad M. Murphy, Karthik Sathiyanadan, Yu Wei, Alaa Othman, Matilde Laurá, Yo-Tsen Liu, Anke Penno, Julian Blake, Michael Donaghy, Henry Houlden, Mary M. Reilly, Thorsten Hornemann

Neuromolecular Med. 2015; 17(1): 47–57. Published online 2015 Jan 8. doi: 10.1007/s12017-014-8339-1

PMCID: PMC4326654

Epidermal Snail expression drives skin cancer initiation and progression through enhanced cytoprotection, epidermal stem/progenitor cell expansion and enhanced metastatic potential

B De Craene, G Denecker, P Vermassen, J Taminau, C Mauch, A Derore, J Jonkers, E Fuchs, G Berx

Cell Death Differ. 2014 Feb; 21(2): 310–320. Published online 2013 Oct 25. doi: 10.1038/cdd.2013.148

PMCID: PMC3890953

Thiopental Inhibits Global Protein Synthesis by Repression of Eukaryotic Elongation Factor 2 and Protects from Hypoxic Neuronal Cell Death

Christian I. Schwer, Cornelius Lehane, Timo Guelzow, Simone Zenker, Karl M. Strosing, Sashko Spassov, Anika Erxleben, Bernd Heimrich, Hartmut Buerkle, Matjaz Humar

PLoS One. 2013; 8(10): e77258. Published online 2013 Oct 22. doi: 10.1371/journal.pone.0077258

PMCID: PMC3805597

Hereditary sensory and autonomic neuropathy type 1 (HSANI) caused by a novel mutation in SPTLC2

Sinéad M. Murphy, Daniela Ernst, Yu Wei, Matilde Laurà, Yo-Tsen Liu, James Polke, Julian Blake, John Winer, Henry Houlden, Thorsten Hornemann, Mary M. Reilly

Neurology. 2013 Jun 4; 80(23): 2106–2111. doi: 10.1212/WNL.0b013e318295d789

PMCID: PMC3716354