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DMEM:F12, 1:1 Mix with stable glutamine, with HEPES - 500 mL

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  • Competent technical support +49(0)731-3608-123

€13.74*

Available in 1 day, delivery time 3 days

Volume
Product number: C4071.0500
Delivery time 3 days

Shipment: not cooled. Store at +2°C to +8°C. For laboratory usage only!
Delivery time 3 days
Product information "DMEM:F12, 1:1 Mix with stable glutamine, with HEPES - 500 mL"

DMEM:F12, 1:1 mixture without L-glutamine, with 15mM Hepes buffer and 1.2/L NaHCO3. Can be supplemented by adding 7% NaHCO3 (> C4213) and/or stable glutamine (> C4282) and thus adapted to the corresponding special applications.

DMEM/F-12 is a 1:1 mixture of Dulbecco's Modified Eagle Medium and Ham's F-12. It combines the high levels of glucose, amino acids and vitamins from DMEM with the various components of Ham's F-12. It is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.

DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10% Fetal Bovine Serum (FBS). DMEM/F-12 uses a sodium bicarbonate buffer system and therefore requires a 5–10% CO2 environment to maintain physiological pH.

Genaxxon offers a variety of DMEM modifications for a range of cell culture applications. If you can't find the medium you are looking for we will be glad to prepare the requested medium you do need. Just let us know your needs: info@genaxxon.com.

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Specification:
without L-Glutamine
with Glucose
with Phenol red
with Sodium pyruvate
with HEPES
with 1.2g/L NaHCO3

sterile filtered solution for cell culture


Applikation / Application:
For use in cell culture applications. Can be supplemented by adding 7% NaHCO3 (> C4213) and/or stable glutamine (> C4282) and thus adapted to the corresponding special applications.

Einheiten / Units:
Quelle / Source:


Sicherheits Hinweise / Safety


Klassifizierungen / Classification

eclass-Nr: 32-16-08-01
Documents - Protocols - Downloads :
Here you will find information and further literature. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: info@genaxxon.com or phone: +49 731 3608 123.


Documents:

Protocols
General Data 1
Listed below are articles and references, in which the authors trust in the high quality of this Genaxxon product.
Source: NCBI PubMed