Stable Glutamine, 200mM (100-times)
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200mM L-Alanyl-L-Glutamine solution (100-times) (stabile Glutamine) is a ready-to-use, sterile 200mM cell culture media supplement.
The concentration of L-glutamine used in classical media ranges from 0.5mM in Ames' Medium to 10mM in MCDB Media 131. The more typical concentrations in media used is between 2 and 6mM. DMEM/F12 (50:50) is often used as a starting formulation for media used with Chinese Hamster Ovay, CHO, cells. L-glutamine in DMEM/F12 is 2.5mM. L-glutamine in DMEM, GMEM, IMDM and H-Y medium is 4mM.
Normal L-Glutamin > is not stable in solution. A drawback of the spontaneous breakdown once in solution is the generation of ammonia as a by-product, which is toxic to the cells and can affect protein glycosylation. One strategy to minimize generation of ammonia is to replace the L-glutamine in medium with stable glutamine (L-Alanyl-L-Glutamine) from Genaxxon. This supplement shows an improved stability compared to free L-Glutamine and considerable reduction of spontaneous ammonia generation.
Specifications:
200 mM sterile filtered solution of stable glutamine (L-Alanyl-L-Glutamine).
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
eclass-Nr: 32-19-90-90
Documents:
Spec. Product DescriptionSource: NCBI PubMed
