Ponceau S (C.I. 27195)
Order number: M3349.0010Shipping: shipped and stored at RT
Delivery time: 3- 8 working days
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Ponceau S is used for reversible staining proteins blotted on nitrocellulose membran and while running a TAU electrophoresis. The reversibility of the staining process is ideally suited for subsequent immunoblots (western blot) or other colour reactions as Ponceau S can be completely removed. Ponceau S is not stable for long time, thus proteins and protein markers should be marked with a pencil. For staining the membrane is incubated in a 0.1% Ponceau S solution (w/v) in 5% acetic acid for 5 - 10 minutes. Destaining is done by incubating the stained membrane in 5% acetic acid for 15 minutes.
Assay: min. 80%
Appearance: red to brown powder
Loss on drying: max. 10%
λ(max) (water): 517-523nm
MW = 760.56 g/mol
application:for reversible staining of blotted membranes
Sicherheits Hinweise / Safety
Klassifizierungen / ClassificationEC-Nr: 228-319-2
Dokumente - Protokolle - Downloads
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