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Orange G (C.I. 16230) can be used as tracking dye for gel electrophoresis.
To stop the electrophoretic mobility-shift assay, 5μL of a 10% glycerol / 0.01% orange G mixture is added to 20μL sample volume. The samples were then applied directly to a 5% polyacrylamide gel (40: 1). In agarose gel electrophoresis, Orange G is used as a tracking dye. Since Orange G "runs" at about 20bp to 40bp, it does not cover DNA bands in the range larger than 60bp.
Assay (UV): min. 90%
Appearance: dark orange to red powder
Solubility (water, 20°C): 70 g/L
pH (1%, water): 9.0 - 10.0
λmax. (H2O): 476-481nm
Loss on drying: max. 10%
MW = 452.38 g/mol
Sicherheits Hinweise / Safety
Klassifizierungen / ClassificationEC-Nr: 217-705-6
Dokumente - Protokolle - Downloads
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