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The mixed isomers 5/6-FAM (5/6-Carboxyfluorescein) is an amine-reactive derivative of Fluorescein > giving stable derivatives upon conjµgation with biopolymers. The amine-reactive fluorescein derivatives 5-FAM > and 6-FAM > and the mixed isomers form 5/6-FAM > are the most common fluorescent derivatizing reagents for the covalently labeling of proteins. Carboxyfluorescein can be retained much better in cells than fluorescein since its pKa is lower than the cytosolic pH of most cells. 6-FAM contains a carboxylic acid which can react with primary amines via carbodiimide activation. Carboxyfluorescein is commonly used as a polar tracer.
Other fluorescent dyes from Genaxxon: TAMRA >, ROX >, DAF-2 >
Purity: ≥95% (Titr.)
Identity: Determined by NMR/IR
Appearance: orange powder
Soluble in: DMSO
MP = 275°C
MW = 376.32 g/mol
Fluorescence: λex 492 nm; λem 517 nm in 0.1M Tris pH8.0
application:fluorescent dye for labelling of proteins
Sicherheits Hinweise / Safety
Klassifizierungen / ClassificationCAS-Nr: 72088-94-9
Dokumente - Protokolle - Downloads
Here you will find information and further literature on 5/6 FAM mixed isomers. For further documents (certificates with additional lot numbers, safety data sheets in other languages, further product information) please contact Genaxxon biosience at: email@example.com or phone: +49 731 3608 123.