6-FAM, 6-Carboxyfluorescein - single isomer
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Shipment: not cooled. Store at +2°C to +8°C. For laboratory usage only!
The single isomer 6-FAM (6-Carboxyfluorescein) is an amine-reactive derivative of Fluorescein > giving stable derivatives upon conjugation with biopolymers. The single amine-reactive fluorescein derivatives 5-FAM > and 6-FAM > and the mixed isomers form 5/6-FAM > are the most common fluorescent derivatizing reagents for the covalently labeling of proteins. Carboxyfluorescein can be retained much better in cells better than fluorescein since its pKa is lower than the cytosolic pH of most cells. 6-FAM contains a carboxylic acid which can react with primary amines via carbodiimide activation. Carboxyfluorescein is commonly used as a polar tracer.
Other fluorescent dyes from Genaxxon: TAMRA >, ROX >,DAF-2 >
Specifications:
Purity: ≥98% (HPLC)
Identity: Determined by 1H-NMR
Appearance: yellow powder
Soluble in: DMSO
Formula: C21H12O7
MP = >300°C
MW = 376.32 g/mol
Fluorescence: λex 492 nm; λem 517 nm in 0.1M Tris pH8.0
Sicherheits Hinweise / Safety
Klassifizierungen / Classification
CAS-Nr: 3301-79-9eclass-Nr: 30-19-60-90
Documents:
CertificateGeneral Data 1
Category List
Source: NCBI PubMed
