5-Carboxyfluorescein succinimidyl ester / 5-FAM-SE



Order number: S5423.0020

Shipping: shipped at RT, store at +2°C to +8°C
CAS-Nr: 92557-80-7

Delivery time: 3- 8 working days
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€104.39 *

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5-Carboxyfluoresceinsuccinimidylester. Amine-reactive fluorescein dye marker for fluorescence... more
Product information "5-Carboxyfluorescein succinimidyl ester / 5-FAM-SE"

5-Carboxyfluoresceinsuccinimidylester. Amine-reactive fluorescein dye marker for fluorescence labeling, especially for labeling of biomolecules (Abs: 495 nm, Em: 519 nm). This single isomer amine-reactive derivatization reagent may be used for covalent labeling of proteins and alkylamino modified nucleic acids. Fluorescein has an excitation maximum that closely matches the 488nm spectral line of the argon-ion laser, making it the predominant fluorophore for confocal laser scanning microscopy and flow cytometry applications. It has a relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility. Importantly, protein-fluorescein conjugates are not inordinately susceptible to precipitation. The coupling reaction is usually carried out at pH 8-9.5.

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Specifications: Assay: min. 95% Identity: 1H-NMR Appearance: Yellow to orange powder... more
 

Technical Data:

Specifications:
Assay: min. 95%
Identity: 1H-NMR
Appearance: Yellow to orange powder
Fluorescence: λex 492 nm, λem 520 nm in 0.1M Tris, pH 8.0
Density: 1.74 g/cm3
C25H15NO9
MW=473.39 g/mol

application:

fluorescent dye for labelling of proteins

Source

synthetic

Sicherheits Hinweise / Safety

Klassifizierungen / Classification

CAS-Nr: 92557-80-7
eclass-Nr: 39-21-01-90
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