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Carbocyanine dyes, particularly thiacyanines such as DiSC3(3) or DiSC3(5) can inhibit respiration and may therefore be relatively cytotoxic. 3,3'-dipropyl-thiadicarbocyanine iodide is sensitive to membrane potential, fluorescence response to depolarization depends on the staining concentration and detection method. Selected applications are: Calcium channels and other ion transport systems, mitochondrial activity, neurons and brain tissue, membrane potential in intact yeast cells.
Potential-sensitive fluorescent dye used in fluorescence microscopy or fluorimetric measurements. DiSC3(5) is a well established reporter of membrane potential in bacteria.
Potential-sensitive fluorescent dye
Assay: min. 98% (NMR); Identity determined by: NMR; C23H25lN2S2; MW = 520.49 g/mol; Soluble in: MeOH, DMF, DMSO; MP = 288°C; Fluorescence: λex 556 nm; λem 575 nm in methanol.
application:used in fluorescence microscopy or fluorimetric measurements
Sicherheits Hinweise / Safety
Klassifizierungen / ClassificationCAS-Nr: 53336-12-2