5/6 FITC / Fluorescein 5(6)-isothiocyanate

Fluorescein 5/6-isothiocyanate mixture of 5 and 6 Fluorescein-isothiocyanate. Reagent for the FITC labeling of proteins, Microsequencing of proteins and peptides (HPLC). Biological applications include use as a fluorescent labeling reagent for proteins, a fluorescent reagent for protein tracing, and a reagent in the fluorescent antibody technique for the rapid identification of pathogens.

FITC is yellow-orange in color with an absorption maximum at 495 nm. Upon excitation it emits a yellow-green color with an emission maximum at 525 nm. FITC reacts under mild conditions with primary amines. Used in modification of actin at lys-61, Labelling of FAB and the modification of Thiol groups.